Fluorochrome Spectrum Change Detection for Flow Cytometry
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Solution Overview
Problem
The state of fluorescent reagents can change over time due to factors like light, oxidation, and temperature, leading to inaccurate panel design and unmixing processing in flow cytometry, especially for tandem reagents stored in different bottles, where past master data may not reflect the current state.
Innovation Solution
An information processing device and system that detect changes in single stain spectrum information of fluorochromes by comparing current and reference data, generating alerts, and prompting updates to ensure accurate panel design and unmixing processing.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If master data or past spectrum information is used for fluorochrome analysis, then processing efficiency is improved, but measurement precision deteriorates due to reagent state changes over time
Solution Approach 1:
The system performs preliminary measurement of the fluorochrome spectrum before panel design or unmixing processing. This preliminary action captures the current state of the fluorochrome reagent, ensuring that the most up-to-date spectral information is used for subsequent analysis, thereby resolving the contradiction between using past data for efficiency and current data for precision.
Solution Approach 2:
The system implements feedback by comparing the currently measured fluorochrome spectrum with previously stored reference data. This feedback mechanism detects changes in the fluorochrome state over time and triggers updates to the master data, ensuring that panel design and unmixing processing always use accurate, current spectral information rather than outdated data.
2Measurement precision
If spectrum information is updated frequently to reflect current reagent state, then measurement precision is improved, but loss of time increases due to repeated measurements
Solution Approach 1:
The system uses feedback to intelligently determine when updates are necessary. By comparing current measurements with stored reference data and detecting significant changes, the system updates master data only when needed, avoiding unnecessary repeated measurements while ensuring precision when reagent state actually changes.
Solution Approach 2:
The system monitors changes in spectral parameters (wavelength, intensity) of the fluorochrome over time. By tracking these parameter changes and comparing them against thresholds, the system determines when reagent deterioration has occurred and updates are required, balancing measurement precision with time efficiency.
3Device complexity
If panel design is performed using outdated fluorochrome data, then device complexity is reduced, but reliability deteriorates due to inaccurate analysis results
Solution Approach 1:
The system performs preliminary measurement and validation of fluorochrome spectrum data before it is used in panel design or unmixing processing. This ensures that the data fed into these complex processes is current and accurate, improving reliability without adding significant complexity to the overall system architecture.
Solution Approach 2:
The system implements feedback loops that verify the quality and currency of fluorochrome spectral data before allowing panel design or unmixing operations to proceed. This feedback mechanism ensures reliability by preventing the use of outdated data while maintaining manageable system complexity through automated validation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate panel design and unmixing processing by using up-to-date fluorochrome information, improving the reliability of flow cytometry results.
Implementation Method 1
a particle population such as cells, microorganisms, and liposomes is labeled with a fluorochrome, and the intensity and/or pattern of fluorescence generated from the fluorochrome excited by irradiating each particle of the particle population with laser light is measured
Implementation Method 2
The flow cytometer can convert light detected by a photodetector into an electrical signal, quantify the electrical signal, and perform statistical analysis
Data Source
AI summary
An object of the present disclosure is to provide a technique for appropriately grasping the state of a fluorescent reagent. The present disclosure provides an information processing device including a processing unit configured to detect a change in single stain spectrum information of a fluorochrome. The processing unit may compare first single stain spectrum information of the fluorochrome with second single stain spectrum information of the fluorochrome, and determine whether single stain spectrum information changes on the basis of the comparison. The first single stain spectrum information and the second single stain spectrum information may be single stain spectrum information related to the same fluorochrome, and may be acquired at different time points. The single stain spectrum information may be fluorescence intensity spectrum data of fluorescence generated by irradiating the fluorochrome with light.