FumDPS Enzyme Catalysis for Selective Fumonisin Degradation
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Solution Overview
Problem
Existing methods for removing fumonisin, a toxic mycotoxin, face limitations such as chemical safety risks and quality changes in food, while biodegradation offers a mild and specific solution but lacks effective enzymes.
Innovation Solution
Development of a fumonisin degrading enzyme FumDPS, its encoding gene, and recombinant expression vector, enabling efficient degradation of fumonisin using a recombinant strain like E. coli BL21(DE3) with optimal activity at 37°C and pH 8-9.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If chemical methods are used to remove fumonisin, then the content of FB1 is reduced, but new chemicals are introduced and safety risks increase
Solution Approach 1:
The patent converts the harmful effect of chemicals into a beneficial enzymatic process. Instead of using chemical agents that introduce new harmful substances, the invention employs a naturally derived enzyme (FumDPS) that specifically degrades fumonisin through biological catalysis, transforming a harmful toxin into harmless degradation products without introducing additional chemicals.
Solution Approach 2:
The patent replaces chemical methods with a biological enzymatic system. The chemical removal process is substituted by an enzyme-catalyzed degradation pathway where FumDPS protein specifically targets and breaks down fumonisin molecules, eliminating the need for chemical reagents and their associated safety risks.
2Quantity of substance
If physical methods are used to remove fumonisin, then a certain amount of fumonisin is removed, but the quality and flavor of food change
Solution Approach 1:
The patent changes the operational parameters from physical processing to enzymatic catalysis. The enzyme FumDPS operates under mild conditions (optimal temperature and pH) that preserve the chemical integrity of food components, selectively degrading only the fumonisin toxin while leaving the quality and flavor characteristics of the food unchanged.
Solution Approach 2:
The invention converts the potential harm of physical processing (which alters food quality) into a beneficial selective degradation process. The enzyme provides specific recognition and degradation of fumonisin without affecting other food components, transforming a non-selective physical process into a precise biological one that preserves food quality.
3Object-affected harmful factors
If biodegradation method is used to degrade fumonisin, then mild reaction and strong specificity are achieved, but effective enzymes are lacking
Solution Approach 1:
The patent uses molecular cloning technology to copy and amplify the functional gene (fumDPS gene) from the producing organism. The cloned gene is introduced into a host system (such as E. coli) to produce large quantities of the FumDPS enzyme, ensuring reliable and scalable production of this effective degradation enzyme.
Solution Approach 2:
The patent employs a recombinant expression system as an intermediary to produce the enzyme. The fumDPS gene is inserted into an expression vector, which is then introduced into a host organism to facilitate enzyme production. This intermediary system enables reliable, large-scale production of the effective FumDPS enzyme that would be difficult to obtain directly.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
FumDPS effectively degrades fumonisin with high specificity and stability, reducing health risks and maintaining food quality, suitable for agricultural and food applications.
Implementation Method 1
The biodegradation of fumonisin has the advantages of mild reaction, strong specificity and wide application prospect
Implementation Method 2
provide a fumonisin degrading enzyme FumDPS... effectively degrades fumonisin with high specificity and stability
Data Source
AI summary
The present invention relates to the field of genetic engineering, particularly to a fumonisin degrading enzyme FumDPS, gene and application thereof. The present invention provides a fumonisin degrading enzyme FumDPS from Pigmentiphaga sp. which has the amino acid sequence of SEQ ID NO: 1, has the optimum temperature of 37° C., and the optimum pH of 8 to 9, remains more than 60% of the activity in the range of pH 3 to 7, and has 100% of degradation rate of fumonisin B1 at the optimum temperature and pH. The enzyme of the present invention can be applied to reduce the harm of fumonisin to animals and human health in agriculture, feed and food.