Hydrolases TRIZ Cases
- MLH1 Methylation Assay for Reproducible FFPE Cancer Testing
Methylation-sensitive and insensitive enzymes enable a multiplex MLH1 promoter assay that improves reproducibility when testing inhibitor-prone FFPE samples.
- Acyl-CoA Hydrolase Variants for 3-Methylcrotonyl-CoA Conversion
Specific amino acid mutations improve acyl-CoA hydrolase conversion of 3-methylcrotonyl-CoA into 3-methylcrotonic acid for industrial enzymatic production.
- CRISPR HBD Promoter Editing to Increase HbA2 Expression
Instead of focusing mainly on HbF, the case edits the HBD promoter with KLF1, b-DRF, TFIIB, and LDB1 sites to enhance HbA2.
- Protease-Compatible Alpha-Amylase for Low-Temperature Cleaning
Targeted amino acid substitutions help alpha-amylase resist protease degradation while maintaining starch-removal activity at low temperatures.
- Thermostable Esterase Variants for Industrial Polyester Degradation
Thermostable esterase variants use targeted amino acid changes and structural bridges to support industrial hydrolysis of polyester materials.
- Urethanase–Lipase Hydrolysis for Defined Polyurethane Recycling
Urethanase polypeptides target urethane bonds while lipases hydrolyze ester groups, enabling polyurethane recycling at mild temperatures.
- PET-Degrading Esterase Variants With Targeted Amino Acid Changes
Targeted substitutions in esterase variants improve PET hydrolysis activity and thermostability, helping address inefficient plastic waste degradation.
- Cas9/gRNA Transfection of Grapevine Protoplasts for Low-Stress Regeneration
PEG-mediated Cas9:gRNA transfection uses dark incubation, saline washing, and minimal centrifugation to reduce protoplast stress during grapevine regeneration.
- HDR Gene Editing in HSPCs for Precise Haploinsufficient-Gene Knock-In
Growth-factor priming moves HSPCs toward HDR-permissive phases, while outcome purging supports precise, safer knock-in.
- PI-, PC-, and PE-Specific Phospholipase C for Low-Loss Degumming
Existing degumming can lose oil through phospholipid emulsification; targeted phospholipase C activity removes major phospholipid classes more efficiently.
- Phage-Derived Particles for In Situ C. Acnes Gene Delivery
Phage packaging signals help deliver DNA into C. acnes in situ, overcoming electroporation’s in vitro limitation.
- gRNA Scaffold Screening for Stable CRISPR Multiplexing
Function and processing screens identify gRNA scaffolds that reduce homologous repeats, supporting stable arrays for simultaneous multi-site CRISPR targeting.
- Cas9 Amino Acid Substitutions for Genome Editing
Cas9 substitutions at residues 86, 98, and 155 address low transformation efficiency while improving genome editing in host cells.
- CRISPR-Cpf1 Paired Guide RNAs for AT-Rich Genomic Deletions
Cpf1 guide RNA arrays target T-rich PAM sites for precise deletions in AT-rich regions while reducing cloning complexity and off-target effects.
- Transient Low-Affinity Probe Kinetics for Specific Protein Detection
Low-affinity probes use transient binding kinetics to separate specific from nonspecific events, improving single-molecule protein detection.
- FumDPS Enzyme Catalysis for Selective Fumonisin Degradation
Chemical removal can introduce safety risks, while physical processing may alter food quality; FumDPS enables mild, specific fumonisin degradation.
- Modular Cas13d CRISPR Effectors for Diverse Nucleic Acid Editing
Limited CRISPR effector diversity is addressed with modular Cas13d proteins supporting RNA cleavage, base editing, and regulation.