Fumonisin Esterase Variants for Heat-Stable Mycotoxin Detoxification
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing fumonisin esterases exhibit limited enzymatic activity across a broad range of temperatures and pH levels, restricting their application in various processing conditions.
Innovation Solution
Development of polypeptides with modified amino acid sequences, such as SEQ ID NO: 1, 2, or 33, exhibiting increased fumonisin esterase activity, particularly at elevated temperatures, and capable of hydrolyzing tricarballylic acid moieties in fumonisins, including variants and fragments with at least 70% identity to these sequences.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If existing fumonisin esterases are used, then fumonisin degradation activity is achieved, but enzymatic activity is limited across broad temperature and pH ranges
Solution Approach 1:
The patent applies parameter changes by modifying amino acid residues at specific positions (1, 33, 87, 129, 136, 173, 265, 269, 284, 287, 295, 313, 314, 327, 374, 376, 384, 389, 424, 430, 468, 482) in the fumonisin esterase sequence to create variants with improved stability and activity across broader temperature and pH ranges. These sequence modifications enable the enzyme to maintain reliable degradation activity under diverse processing conditions.
Solution Approach 2:
The patent applies local quality by making specific localized amino acid substitutions at defined positions in the enzyme sequence while maintaining the overall structure. This targeted modification approach allows the enzyme to gain enhanced adaptability to different temperatures and pH levels while preserving its core fumonisin degradation function.
2Object-affected harmful factors
If fumonisin esterase is applied for detoxification, then fumonisin toxicity is reduced, but processing options are restricted due to limited temperature and pH stability
Solution Approach 1:
The patent modifies the enzyme's amino acid sequence to change its physical-chemical parameters, specifically improving thermal stability and pH tolerance. This enables the enzyme to function reliably in downstream processing operations that involve heat treatment or pH adjustment, thereby expanding processing options while maintaining detoxification efficacy.
Solution Approach 2:
The patent creates dynamic adaptability by engineering enzyme variants that can maintain functionality under varying processing conditions. The modified enzyme can adapt to different temperature and pH environments encountered in various downstream processing steps, making the detoxification process more flexible and versatile.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The modified polypeptides demonstrate enhanced stability and activity under heat challenges, enabling effective detoxification of fumonisins in diverse applications, including food, feed, and beverage ingredients.
Implementation Method 1
The two tricarballylic acid moieties of FB1 can be sequentially cleaved off by esterases, which results in two intermediate products, partially hydrolyzed FB1-1 and 2 (namely pHFB1-1 and pHFB1-2, respectively).
Implementation Method 2
Fumonisin esterases do not necessarily exhibit enzymatic activity across a broad range of temperatures and pH which limits downstream processing options and applications.
Data Source
AI summary
The present disclosure concerns polypeptide having fumonisin esterase activity exhibiting increased fumonisin esterase activity, when measured at a temperature of at least 37° C., when compared to the control polypeptide comprising the amino acid of SEQ ID NO: 3. The polypeptides of the present disclosure include one or more amino acid variations which contribute to the increase fumonisin esterase activity. The present disclosure also includes compositions comprising the polypeptide having fumonisin esterase activity, methods for detoxifying a fumonisin mycotoxin as well as processes for making the compositions comprising the polypeptide having fumonisin esterase activity.