Hydrolases TRIZ Cases

Targeted PGA mutations improve stability, activity, and selectivity during immobilization for 6-APA production and insulin-dimer deprotection.

CRISPR guide RNAs selectively disable the mutant ELANE allele to restore neutrophil production while avoiding risks linked to G-CSF or donor transplant.

Specific lipase sequence changes preserve detergent activity at low calcium levels and improve lipid stain removal despite ingredient interference.

High-pressure molecular dynamics and multi-scale free energy calculation pinpoint hydrolase mutations that raise robustness while cutting screening workload.

Engineered microbial pathways replace plant extraction to produce high-purity mogrosides with better yield and fewer off-flavors.

AAV delivery of TMPRSS3 or LOXHD1 genes helps preserve hair cells and spiral ganglion function, improving cochlear implant outcomes.

Expression levels of FGF2, C3, and GIMAP7 classify high-risk nasopharyngeal cancer patients for recurrence monitoring and tailored treatment.

Pulled hair bulbs with dermal sheath tissue enable simpler, less invasive mesenchymal stem cell culture and proliferation for downstream differentiation.

A synthetic microbial enzyme pathway converts hexanoic acid into cis-3-hexenol while avoiding linolenic-acid byproducts and cost barriers.

Active-site and target-binding mutations tune Cas12a2 cleavage to improve RNA detection while limiting collateral dsDNA degradation.

Lower phytase dosing in fish feed hydrolyzes phytate for growth while cutting phosphorus excretion and reducing inorganic phosphate use.

Target-triggered Cas12 ssDNA cleavage turns guide RNA recognition into a sensitive, specific DNA detection signal with fewer added components.

CRISPR sgRNA knockout of CD7 stops CAR-T cell self-killing, enabling universal CD7-targeting cells with lower graft-versus-host risk.

CD73 levels help identify NSCLC patients most likely to benefit from combined CD73 and PD1/PD-L1 blockade, improving survival outcomes.

Complementary pegRNA RT templates enable precise target DNA fragment duplication and amplification without custom long donor designs.

Optimized CRISPR delivery and HDR template design enable precise TSC2 SNP editing in hMSCs while preserving viability and limiting off-target effects.

Surfactant-assisted binding on amino-functional hydrophobic supports avoids activation and animal proteins while preserving lipase activity and stability.

CRISPR cutting creates overhangs that are label-filled and bead-captured to enrich low-frequency nucleic acid targets for better sequencing coverage.

Targeted edits in the crRNA:tracrRNA nexus and stem improve Cas9 binding, sharpen DNA targeting, and reduce off-target editing.

Mono- and disaccharides stabilize liquid deamidase compositions during storage, preserving enzyme activity while keeping pumpable handling.

Specific GFAP-binding antibodies enable point-of-care serum detection across 5 pg/mL to 50,000 pg/mL without dilution for TBI assessment.

RNA-guided editing creates dominant positive or negative alleles through targeted breaks and rearrangements, improving mutation efficiency and tissue specificity.

A non-pathogenic Bacillus β-amylase stays active above 60°C, enabling raw starch saccharification with lower cooling and no gelatinization.

Adding non-templated cytidines or uridines at the 3′ end helps small therapeutic RNAs resist nuclease degradation and last longer in cells.

Amorphized PET is enzymatically depolymerized near its glass transition to avoid recrystallization and recover virgin-quality monomers.

Specific Bacillus subtilis-derived proteins convert ansamitocin P3 to maytansinol without pyrophoric reagents, improving industrial suitability.

Ligand-responsive gRNAs switch Cas9 activity on or off to improve site-specific cleavage and reduce off-target toxicity.

CRISPR screening identifies inhibitory T-cell genes to boost proliferation and help engineered cells resist suppressive tumor microenvironments.

Acid extraction, phytase treatment, and membrane filtration produce oilseed protein isolate with low phytic acid, high purity, and strong solubility.

A warmed slide and cooler siRNA or gRNA composition improve surface adhesion, supporting stable transfection and longer cell viability.

Guide RNAs position dCas13 around polyadenylation sites to reshape 3′UTR length, suppress oncogenic signaling, and improve AR inhibitor response.

Using RPA pre-amplification with CRISPR-Cas12a and guide RNA, this case enables rapid, specific Salmonella serotype detection.

Dual probes and endonuclease-released codes enable sensitive, specific nucleic acid detection without PCR bias or lengthy protocols.

Reduced-homology replacement sequences help plastid homologous recombination bypass DNA repair and high copy number for precise point edits.

High-dose RNase digestion isolates ribosome-protected RNA from small samples, enabling rapid single-nucleotide translation mapping.

Labeled oligonucleotides bind nuclease-created 3′ overhangs to distinguish on-target and off-target DNA cuts without denaturing genomic DNA.

A fluorous agent complex delivers genome-editing molecules directly into plant cells, avoiding callus culture, T-DNA integration, and somaclonal variation.

CRISPR editing of intronic regulatory sequences blocks circRNA backsplicing while preserving linear mRNA production and protein expression.

Enzyme-coated electrodes and a mass transport membrane enable continuous in vivo monitoring of aspartate and asparagine without repeated sampling.

A site-directed integration setup inserts one donor vector at a defined genomic site, cutting random events, clone screening load, and biological noise.

Exogenous myrosinase converts glucosinolates to volatile compounds that mild heat and vacuum remove without damaging meal protein.

Consensus-engineered PLC mutations keep degumming active at 60-85°C, removing phospholipids while avoiding extra heat exchangers.

A single HLA-E/HLA-G transgene in iPSCs reduces edit burden while helping allogeneic cell therapies resist NK attack and limit immune risk.

Ribosome occupancy patterns reveal hard-to-find uORFs, enabling precise editing to boost downstream expression and plant traits.

Modified fumonisin esterase sequences improve activity at elevated temperature and broader pH conditions for food and feed detoxification.

Controlled cell permeabilization and target-linked barcodes enable single-cell labeling of nuclear target DNA for chromatin, genome, and methylome sequencing.

Segmented crRNA-tracrRNA with Cas Type V and lipid nanoparticles improves gene editing precision, delivery, and cost-effectiveness.

Cyclization and rolling circle amplification improve integration site mapping sensitivity, enabling accurate detection of low-frequency insertions.

This case shows how designer DNA recombining enzymes edit or replace genomic sequences without relying on host repair pathways that cause random indels.

Engineered esterase variants address low activity and thermostability limits in PET degradation, producing recyclable monomers and oligomers.