Ginger Extract Polysaccharide Composition for Immune Modulation
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Solution Overview
Problem
Current strategies to modulate the immune system, whether natural or synthetic, often fail to effectively support and stimulate immune function, leading to dysregulation and various diseases, and there is a need for more effective immunomodulatory agents, particularly from natural sources.
Innovation Solution
A composition comprising ginger extract polysaccharides is administered to subjects to stimulate and support the immune system, utilizing pathways like TLR-4-MAPK/NF-kB signaling to enhance immune responses.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If current immunomodulatory strategies (natural or synthetic) are used to activate or suppress the immune system, then immune response modulation is attempted, but the effectiveness is insufficient leading to immune dysregulation and disease
Solution Approach 1:
The patent extracts polysaccharides specifically from ginger root through a multi-step process involving extraction with solvents like ethanol or methanol, followed by purification steps including precipitation and centrifugation. This extraction isolates the immunomodulatory active components from the whole plant material, concentrating the beneficial effects while removing inactive or potentially harmful substances.
Solution Approach 2:
The patent optimizes various parameters including solvent concentration (ethanol or methanol at specific percentages), extraction temperature, pH levels during purification, and concentration ratios to maximize polysaccharide yield and immunomodulatory activity. These parameter changes ensure the extract achieves effective immune system modulation without causing dysregulation.
2Reliability
If plant polysaccharides are utilized in dietary supplements and health products, then immune support and pathogen defense are enhanced, but the specific efficacy and mechanism need to be established
Solution Approach 1:
The patent employs multiple assay methods to evaluate immunomodulatory activity, including measurement of cytokine production (TNF-α, IL-6, IL-10), nitric oxide levels, and cellular responses. These feedback mechanisms allow characterization of the polysaccharide extract's effects on immune cell function, providing quantitative data on efficacy while elucidating mechanisms of action.
Solution Approach 2:
The patent utilizes colorimetric assays to detect and quantify immunomodulatory effects, such as measuring nitric oxide production through color changes in Griess reagent assays, and detecting cytokine levels through ELISA colorimetric readouts. These visual indicators provide straightforward assessment of immune system response to the polysaccharide extract.
Data Source
AI summary
The invention provides an immunomodulatory composition comprising an extract of Zingiber officinale root containing one or more biologically active polysaccharides. The composition finds use in methods of supporting and maintaining health and the immune system.