Glucose Oxidase Mutants Balancing Thermal Stability and Activity

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Solution Overview

Problem

Existing glucose oxidase mutants, such as GOD-M5, exhibit low yield and low enzyme activity, limiting their industrial application despite improved thermal stability, which does not meet industrial requirements.

Innovation Solution

Development of glucose oxidase mutants GOD-M6, GOD-M7, GOD-M8, GOD-M9, and GOD-M10 through specific amino acid substitutions, along with the corresponding genes and recombinant vectors and strains, to enhance thermal stability and enzyme activity.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Stability of the object's composition

If glucose oxidase mutant GOD-M5 is used to improve thermal stability, then thermal stability is improved, but enzyme activity and yield remain low

Engineering Contradiction:

Improvethermal stabilityVSAvoidenzyme activity

Core Design Contradiction:

Stability of the object's compositionVSProductivity

Solution Approach 1:

The patent applies parameter changes by introducing multiple amino acid substitutions at specific positions (Asp68Lys, Thr274Phe, Tyr278Thr, Ser94Ala, Thr31Val, Gln88Arg) to modify the enzyme's structural and functional parameters, thereby simultaneously improving both thermal stability and enzyme activity

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent creates a composite enzyme structure by combining multiple mutation effects into a single enzyme variant, where the cumulative effect of multiple amino acid changes produces an enzyme that exhibits both enhanced thermal stability and improved catalytic activity

Inventive Principle:

Principle #40Composite materials

2Stability of the object's composition

If glucose oxidase mutant GOD-M5 is used to improve thermal stability, then thermal stability is improved, but yield remains low

Engineering Contradiction:

Improvethermal stabilityVSAvoidyield

Core Design Contradiction:

Stability of the object's compositionVSQuantity of substance

Solution Approach 1:

The patent modifies the enzyme's structural parameters through multiple amino acid substitutions, which optimizes both the stability and production yield of the enzyme, addressing the limitation of previous mutants

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The mutants exhibit improved thermal stability and enzyme activity, addressing the limitations of previous mutants and enhancing their suitability for industrial applications.

Implementation Method 1

glucose oxidase can oxidize glucose to produce D-gluconolactone in the presence of molecular oxygen

Methodology Applied

Scientific EffectOxidation: Oxidation

Implementation Method 2

GOD specifically catalyzes the substrate β-D-glucose to produce gluconic acid and hydrogen peroxide with oxygen

Methodology Applied

Scientific EffectEnzyme catalysis: Enzyme

Implementation Method 3

catalase can decompose hydrogen peroxide into water

Methodology Applied

Scientific EffectCatalysis: Catalysis

Data Source

PatentUS12522806B2Mutant glucose oxidase (god) having improved thermal stability and gene and application thereof

Publication Date: 2026.01.13 FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES

AI summary

The present invention relates to the field of genetic engineering, particularly to a glucose oxidase mutant having improved thermal stability, gene and application thereof. The present invention provides several glucose oxidase GOD mutants with high catalytic efficiency and improved thermal stability, which breaks the barrier of low enzyme activity and poor stability and is suited well to meet the requirements of application to the fields of food, medicine, feed and textile industry, and has a very broad application prospect.