Transformed Cell Lines Expressing GPCR22 for Guanosine Screening

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Solution Overview

Problem

The mechanism through which guanosine exerts its biological effects is unclear, and there is a lack of understanding regarding the specific receptor it interacts with, hindering the identification of guanosine analogues and the determination of cell types expressing this receptor.

Innovation Solution

Identification of G-Protein Coupled Receptor 22 (GPCR22) as a novel guanosine binding receptor, with methods involving transfection of host cells to express GPCR22 and use biochemical, pharmacological, and physiological techniques to confirm its binding and functional effects, allowing for the identification of guanosine analogues and responsive cell types.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If transfection methods are used to express GPCR22 in host cells, then the ability to screen guanosine analogues and identify responsive cell types is improved, but the complexity of the experimental system increases

Engineering Contradiction:

Improveability to screen guanosine analoguesVSAvoidcomplexity of experimental system

Core Design Contradiction:

Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent uses GPCR22 as an intermediary receptor to mediate the interaction between guanosine and host cells. By transfecting GPCR22 into host cells that do not naturally express it, the system enables specific guanosine binding and downstream signaling, allowing for effective screening of guanosine analogues while maintaining a relatively simple experimental setup using established transfection protocols

Inventive Principle:

Principle #24Intermediary (Mediator)

2Measurement precision

If biochemical and pharmacological techniques are used to confirm receptor binding, then the precision of receptor identification is improved, but the time and resources required increase

Engineering Contradiction:

Improveprecision of receptor identificationVSAvoidtime required for confirmation

Core Design Contradiction:

Measurement precisionVSLoss of time

Solution Approach 1:

The patent performs preliminary characterization of GPCR22 binding properties and functional effects before conducting comprehensive screening assays. By establishing the receptor's binding specificity and downstream effects in advance, the research team creates a validated platform that accelerates future analogue screening while maintaining high identification precision through established biochemical assays

Inventive Principle:

Principle #10Preliminary action

3Productivity

If transformed cell lines are developed for screening, then the productivity of analogue identification is improved, but the manufacturing complexity increases

Engineering Contradiction:

Improveproductivity of analogue identificationVSAvoidease of creating transformed cell lines

Core Design Contradiction:

ProductivityVSEase of manufacture

Solution Approach 1:

The patent develops transformed cell lines expressing GPCR22 that serve multiple functions: they can screen guanosine analogues, identify responsive cell types, and characterize receptor signaling pathways. This multi-functional cell line platform increases productivity across different research objectives while utilizing standard transfection and culture techniques that minimize manufacturing complexity

Inventive Principle:

Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

GPCR22 is verified to bind guanosine and elicit cellular effects, enabling the development of transformed cell lines for screening guanosine analogues and identifying cell types capable of binding guanosine, advancing the understanding of guanosine's biological pathways and potential therapeutic applications.

Implementation Method 1

GPCR22 is a cell-surface G-Protein Coupled Receptor able to bind guanosine and guanosine analogs

Methodology Applied

Scientific EffectG-Protein Coupled Receptor binding:

Implementation Method 2

The host cell is transfected with a recombinant cDNA sequence coding for an amino acid sequence of the cell-surface G-Protein Coupled Receptor

Methodology Applied

Scientific EffectRecombinant protein expression:

Data Source

PatentUS10520491B2Transformed cell lines expressing non-endogenous cell-surface selective guanosine-responsive G-protein coupled receptors

Publication Date: 2019.12.31 LIBRAMEN NATURALS

AI summary

Disclosed herein are cell lines transformed to express G-Protein Coupled Receptor GPCR22 and uses thereof for identifying guanosine analogues and/or other ligands to the receptor. In particular, techniques for transforming Drosophila Schneider 2 cells and human astrocytoma 1321N1 cell to express GPCR22 are disclosed as well as transformed cells lines. The transformed cell lines of the instant disclosure may be useful in identifying guanosine analogues and functional equivalents thereof.