Holographic Scatterer Localization via Intensity Gradient Transforms

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Solution Overview

Problem

Conventional image analysis algorithms face challenges in accurately and efficiently identifying the position of small scatterers in holograms due to the complexity of fitting measured holograms to theoretical predictions, particularly with small objects having alternating bright and dark fringes in the field of view.

Innovation Solution

The method employs intensity gradients to locate the center of scatterers using a continuous transform of the local field, eliminating the need for threshold selection and reducing computational burden, and incorporates machine learning techniques like neural networks and support vector machines to analyze holograms for precise particle tracking and characterization.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If conventional image analysis algorithms are used to fit measured holograms to theoretical predictions, then precise measurements of scatterer position, size and refractive index can be obtained, but the processing time is excessive and real-time analysis is not achieved

Engineering Contradiction:
Improvescatterer position measurement precisionVSAvoidhologram processing time
Core Design Contradiction:
Measurement precisionVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-calculating and storing look-up tables containing theoretical hologram patterns for various particle sizes and refractive indices before actual measurement. During real-time analysis, the measured hologram is rapidly matched against these pre-computed patterns through correlation algorithms, eliminating the need for iterative theoretical fitting during processing. This pre-computation approach maintains measurement precision while reducing processing time to enable real-time colloidal characterization.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent uses copying by creating simplified representations of the complex theoretical fitting process through pre-computed look-up tables that store representative hologram patterns. These tables serve as copies of the full theoretical model for common particle parameters, allowing rapid pattern matching without executing the complete theoretical prediction and fitting algorithm in real-time, thus achieving fast yet accurate scatterer characterization.

Inventive Principle:
Principle #26Copying

2Ease of operation

If conventional voting algorithms are used to locate scatterer centers based on intensity gradients, then scatterer positions can be identified, but the discrete nature of the algorithm creates inefficiencies and requires threshold selection

Engineering Contradiction:
Improvescatterer center localizationVSAvoidcomputational efficiency
Core Design Contradiction:
Ease of operationVSProductivity

Solution Approach 1:

The patent replaces the mechanical voting algorithm approach with a continuous mathematical transform method. Instead of discrete pixel voting with threshold selection, the invention applies a continuous transform (such as a Fourier-based or wavelet-based transform) to the intensity gradient field, which analytically identifies scatterer centers without requiring discrete sampling or threshold parameters. This substitution eliminates the computational inefficiencies of iterative voting while maintaining ease of operation for center localization.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Productivity

If machine learning techniques are used to analyze holograms, then real-time processing on low-power computers is achieved, but the initial training phase requires significant computational resources

Engineering Contradiction:
Improvereal-time processing speedVSAvoidtraining phase energy consumption
Core Design Contradiction:
ProductivityVSUse of energy by stationary object

Solution Approach 1:

The patent applies preliminary action by performing the computationally intensive machine learning training phase offline before deployment. During the training phase, the system learns to map hologram features to particle characteristics using labeled training data. Once trained, the model is saved and deployed to low-power devices where only inference is performed during real-time analysis. This separates the high-energy training computation from the low-energy real-time processing, achieving both real-time speed and energy efficiency in operation.

Inventive Principle:
Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This approach enables rapid and accurate identification of scatterer positions with sub-pixel accuracy, providing real-time insights into colloidal dispersion composition and dynamics, and significantly reduces processing time for holographic video microscopy data.

Implementation Method 1

Holographic microscopy records information about the spatial distribution of illuminated objects through their influence on the phase and intensity distribution of the light they scatter

Methodology Applied
Scientific EffectLight scattering: Scattering

Data Source

PatentUS10983041B2Fast feature identification for holographic tracking and characterization of colloidal particles
Publication Date: 2021.04.20 NEW YORK UNIV
  • US10983041B2 patent drawing
  • US10983041B2 patent drawing
  • US10983041B2 patent drawing

AI summary

A method and system for identification of holographic tracking and identification of features of an object. A holograph is created from scattering off the object, intensity gradients are established for a plurality of pixels in the holograms, the direction of the intensity gradient is determined and those directions analyzed to identify features of the object and enables tracking of the object. Machine learning devices can be trained to estimate particle properties from holographic information.