Immune Cell Targeting Probe for Pre-Targeted Drug Delivery
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Solution Overview
Problem
Current cell-targeting drug delivery systems face challenges such as off-target effects, prolonged circulation of drugs, and high toxicity due to the use of red blood cells, while immune cell-targeting systems have potential to minimize these issues but require improved targeting strategies.
Innovation Solution
Development of an immune-cell-targeting probe using a combination of targeting polypeptides and antibodies with pre-targeting binding handles for precise drug delivery, reducing off-target effects and enhancing targeting affinity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of moving object
If red blood cells are used as drug carriers in cell-targeting drug delivery systems, then circulation time is prolonged, but off-target effects and toxicity increase
Solution Approach 1:
The patent applies pre-targeting strategy by first administering a targeting probe that binds to immune cells in advance, then later administering the drug payload. This preliminary binding step allows the drug to be delivered only to pre-selected target cells, reducing off-target effects while maintaining prolonged circulation benefits
Solution Approach 2:
The patent introduces an immune cell-targeting probe as an intermediary between the drug and the target cells. This probe specifically recognizes and binds to immune cells, serving as a mediator that directs drug delivery precisely to intended targets while avoiding non-specific accumulation in off-target tissues
2Reliability
If traditional cell-targeting drug delivery systems are used, then targeted recognition is achieved, but device complexity and cost increase
Solution Approach 1:
The patent segments the drug delivery system into separate functional components: a targeting probe module that binds to immune cells and a drug payload module. This segmentation allows each component to be optimized independently and simplifies the overall system by eliminating the need for complex integrated targeting mechanisms
Solution Approach 2:
The patent employs antibodies with pre-targeting binding handles that can serve multiple functions: they provide targeted recognition of immune cells, enable pre-targeting strategy implementation, and facilitate drug payload delivery. This multi-functionality reduces system complexity by consolidating multiple roles into a single universal targeting agent
3Object-affected harmful factors
If immune cells are used as drug carriers, then off-target effects are reduced, but targeting precision and affinity need improvement
Solution Approach 1:
The patent creates a composite targeting probe structure combining an antibody with a pre-targeting binding handle. This composite design integrates the high specificity of antibody recognition with the enhanced binding properties of the pre-targeting handle, achieving both reduced off-target effects and improved targeting precision simultaneously
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The probe achieves efficient, targeted drug delivery to immune cells with reduced off-target toxicity and prolonged circulation time, improving therapeutic efficacy and safety.
Implementation Method 1
The combination probe is formed by self-assembly of polypeptides (targeting polypeptides) previously discovered by the inventors that can target immune cells and antibodies prepared from these polypeptides as immune antigens
Implementation Method 2
an antibody or a functional fragment thereof prepared from the targeting polypeptide in (1) as an immunogen, and connected with the pre-targeting binding handle
Data Source
Figure 1A~1B
Figure 2~3C
Figure 4
AI summary
Provided are an immune cell targeting probe, a pre-targeting recognition and drug delivery system based on the probe, and a use thereof. A targeting polypeptide and an antibody do not affect their own recognition effects after modification of a pre-targeting binding handle. The targeting polypeptide and the antibody can be self-assembled to form a combined probe, and the self-assembled probe can enhance the recognition capability to immune cells. Different targeting drug delivery strategies such as pre-targeting secondary delivery can be used on the basis of a difference in steric hindrance of binding of a probe to a target. Therefore, an off-target effect during delivery of toxic drugs is avoided or mitigated, and a use range of the toxic drugs is expanded. Due to the fact that a drug delivery ligand and a target recognition function portion can be independently subjected to chemical modification and storage, the drug delivery ligand can further load various drug molecules or modification groups, and has the advantages of high drug loading capacity, convenient synthesis, controllable product quality, etc., and has wide application in the aspects of immune cell imaging, tracing, screening, analysis and targeting drug delivery.