Incubated-Sample Microscope With Segmented Climate-Controlled Chamber

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Solution Overview

Problem

Existing microscopes face challenges in providing an efficient and space-saving incubation solution that allows easy access to samples while minimizing incubation atmosphere loss and energy consumption.

Innovation Solution

A microscope design with a separated housing section forming a large sample chamber, sealed by a lid, and an interface for connecting an external incubation environment conditioning unit, enabling independent control of environmental conditions within the sample chamber.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Volume of stationary object

If a cage incubator enclosing the entire microscope is used, then a large volume incubation space is provided, but access to the working area for placing or manipulating the sample is impaired and the microscope cannot be equipped in a space-saving manner

Engineering Contradiction:
Improveincubation space volumeVSAvoidaccess to working area
Core Design Contradiction:
Volume of stationary objectVSEase of operation

Solution Approach 1:

The incubation chamber is segmented from the main microscope body, allowing the incubation space to be separated into an independent module that can be accessed separately from the working area, thus providing both large incubation volume and easy sample access

Inventive Principle:
Principle #1Segmentation

2Volume of stationary object

If a stage top incubator enclosing only the sample is used, then space requirements are minimized, but access to the sample is impaired since the sample is surrounded by a sealed box which would have to be opened, thus destroying the incubation atmosphere

Engineering Contradiction:
Improveincubation space volumeVSAvoidaccess to sample
Core Design Contradiction:
Volume of stationary objectVSEase of operation

Solution Approach 1:

The incubation chamber is designed as a separate modular unit with its own sealed environment, allowing the sample to be accessed through the microscope working area while the incubation atmosphere is maintained in the segmented chamber, resolving the conflict between small size and sample accessibility

Inventive Principle:
Principle #1Segmentation

3Volume of stationary object

If a cage incubator is used, then large volume incubation is achieved, but energy and gas consumption is high

Engineering Contradiction:
Improveincubation space volumeVSAvoidenergy and gas consumption
Core Design Contradiction:
Volume of stationary objectVSUse of energy by stationary object

Solution Approach 1:

By segmenting the incubation chamber as a separate modular unit rather than enclosing the entire microscope, the volume requiring climate control is reduced to only the essential sample area, thereby decreasing energy and gas consumption while still providing adequate incubation space

Inventive Principle:
Principle #1Segmentation

4Volume of stationary object

If an integrated incubation chamber is formed by a separated housing section, then a larger incubation room is designed compared to stage top incubators, but the structure becomes more complex

Engineering Contradiction:
Improveincubation room volumeVSAvoidhousing structure complexity
Core Design Contradiction:
Volume of stationary objectVSDevice complexity

Solution Approach 1:

The housing is segmented into separate functional sections (incubation chamber and microscope body) that can be independently designed and assembled, which although creating modular complexity, simplifies manufacturing and maintenance while achieving larger incubation volume

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The incubation chamber is designed as a nested module within the overall microscope housing structure, allowing the larger incubation room to be integrated into the microscope system without proportionally increasing external dimensions, thus managing structural complexity

Inventive Principle:
Principle #7Nested doll (Nesting)

Data Source

PatentEP3904939B1Microscope and system comprising such a microscope for examination of an incubated sample and corresponding method
Publication Date: 2025.09.03 LEICA MICROSYSTEMS CMS GMBH
  • EP3904939B1 patent drawingFigure 1
  • EP3904939B1 patent drawingFigure 2
  • EP3904939B1 patent drawingFigure 3

AI summary

The invention relates to a microscope (100) for microscopic examination of a sample (120) comprising a microscope housing (102) enclosing an illumination optics (118), a microscope stage (116) and an imaging optics (124), an integrated sample chamber (106) located within the microscope housing (102) and formed by a separated housing section (104) within said microscope housing (102), the housing section (104) comprising a lid (109) providing direct access to the microscope stage (116) for placing the sample (120) in the sample chamber (106), wherein said housing section (104) comprises an interface (108) for connection of an external incubation environment conditioning unit (110) to the sample chamber (106), the interface (108) being configured to provide a connection between said external incubation environment conditioning unit (110) and the sample chamber (106), such that the environmental conditions in the sample chamber (106) can be controlled when the external incubation environment conditioning unit (110) is connected to the interface (108).