Insect-Derived Peptide Regulates Plant ABA Hormone Levels
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for regulating plant abscisic acid (ABA) hormones, such as using sodium tungstate, have adverse effects on the environment and human body, and there is a lack of reported peptides that specifically regulate plant ABA hormones based on insect oral secretion proteins.
Innovation Solution
Identification of a 28-amino acid short peptide derived from Plutella xylostella, which interacts with the ABA hormone synthase aba1, thereby increasing ABA content in cruciferous plants, and synergistically affecting jasmonic acid (JA) hormone levels.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If sodium tungstate is used to regulate plant ABA hormones, then ABA content can be reduced, but adverse effects on environment and human body occur
Solution Approach 1:
The patent uses a short peptide (28 amino acids) derived from insect oral secretion instead of sodium tungstate. This peptide is biodegradable and environmentally friendly, eliminating the harmful effects while still achieving regulation of ABA content in plants.
Solution Approach 2:
The patent introduces an intermediary substance (the short peptide) that mediates between the insect oral secretion and the plant ABA synthesis pathway. The peptide interacts with ABA hormone synthase to regulate ABA content without using harmful chemicals like sodium tungstate.
2Adaptability or versatility
If insect oral secretion proteins are used to regulate plant hormones, then new peptide-based method is established, but lack of reported peptides specifically regulating ABA hormones exists
Solution Approach 1:
The patent utilizes the insect's own oral secretion proteins (specifically GSS1 from Plutella xylostella) to regulate plant hormones. The insect's natural proteins are shown to interact with plant ABA synthase, allowing the system to serve itself without external chemical inputs.
Solution Approach 2:
The patent segments the insect oral secretion protein GSS1 to identify the specific 28-amino acid peptide region that interacts with ABA synthase. This segmentation allows for precise identification and application of the active peptide segment.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The peptide significantly enhances plant stress resistance by increasing ABA and JA hormone content, leading to improved insect resistance, as demonstrated by increased mortality of Plutella xylostella larvae feeding on treated plants.
Implementation Method 1
a plant protein zeaxanthin epoxidase aba1 that interacts with the GSS1
Implementation Method 2
The ABA also has an inhibitory effect on cell elongation. Meanwhile, under abiotic stress conditions, plants can improve stress resistance by accumulating ABA
Data Source
AI summary
In the present disclosure, a yeast interactive plasmid of a detoxifying enzyme glucosinolate sulfatase (GSS1) is constructed based on a yeast two-hybrid system and then screened with an Arabidopsis thaliana-yeast library, and a plant protein zeaxanthin epoxidase aba1 that interacts with the GSS1 is obtained for the first time. A zeaxanthin epoxidase gene, also known as an aba-1 gene, is an abscisic acid (ABA) synthase that can catalyze oxidative conversion of the zeaxanthin into all-trans violaxanthin, thereby generating carotenoid precursors required in an ABA biosynthetic pathway. Furthermore, a core peptide segment that interacts with the aba1 is obtained by continuously reducing the length of a GSS1 sequence and conducting one-to-one yeast two-hybrid verification with the aba1 sequence.