Hydrolases TRIZ Cases

Dual guide RNA systems paired with single-stranded oligodeoxyribonucleotides enhance homology-directed repair while reducing off-target binding.

CRISPR-Cas9 edits the fea3 genomic locus to modulate plant meristem development, resolving limited control over kernel row number and yield.

Compartmenting peptides form droplet bodies that sequester terpenes, overcoming product inhibition and increasing yield without affecting cell growth.

Segmented enzymatic hydrolysis of low-starch oat suspensions resolves microbiological contamination risks while developing favorable umami flavor profiles.

A split intein vector system assembles functional endonucleases to excise mutated exons from the dystrophin gene.

Inactive RuvC Cas nickase coupled with a base editor achieves high-specificity nucleic acid modification without double-strand breaks.

Tailspike protein P825Orf2 targets Listeria monocytogenes to resolve detection specificity issues and reduce false positives in food safety testing.

A thermostable RNA polymerase performs in vitro transcription reactions across a 30°C to 80°C temperature range.

GmFAT mutant soybean lines accumulate high oleic acid and reduce saturated fatty acids to improve oxidative stability.

Modifying plant genes to recruit RNA-dependent RNA Polymerase generates amplifiable dsRNA, resolving low silencing efficacy in pest control.

A Type II Cas protein-gRNA complex binds target nucleic acids to shield them from external treatments during isolation.

Nuclease treatment degrades dead cell DNA within the silicate matrix, resolving biofilm contamination issues in microbial analysis.

Plant-derived enzymes replicate porcine hydrolysis to achieve hypoallergenicity while ensuring Halal compliance for infant formulas.

A catalytically inactive RNA-guided endonuclease forms an R loop to expose nucleobases for direct deamination.

Compatible cohesive ends from homing endonuclease and restriction enzyme sites allow modular cloning of multiprotein complexes without recleavage.

Non-urease proteins stabilize urease in biocementation, reducing ammonium chloride byproducts while achieving 0.5 MPa compressive strength.

Simultaneous editing of multiple genes in vertebrate cells using targeted nucleases and homology-directed repair templates.

Host IGF1R knockout increases human donor cell contribution in chimeric organs, addressing low regeneration rates.

Double strand-specific nuclease cleaves wild-type DNA to enrich mutant alleles, resolving detection masking by excess wild-type sequences.

Codon-optimized nucleic acid constructs encoding base editors resolve low editing efficiency in maize and wheat.

Compact B-GEn.7 nuclease overcomes viral vector size limits while maintaining high cleavage activity and specificity.

CRISPR/Cas9 editing system detects and modifies nucleic acid molecules containing unnatural nucleotides.

A CRISPR/Cas12a colorimetric sensor system uses urease amplification to detect pathogens via visible color changes.

Targeted antigen-binding proteins inhibit Arginase II to restore T-cell proliferation while avoiding toxicity from non-specific Arginase I interference.

Engineered pegRNAs use appended structural motifs to enhance stability and binding affinity.

Segmented multi-domain enzymes maintain hydrolytic function in oxidizing agents, resolving stability limitations of single-domain variants.

PDE5A destabilizing domains fused to target proteins recruit the ubiquitin-proteasome system for rapid knockdown, bypassing slow transcriptional delays.

Hydrophobically modified polyvinyl alcohol particles encapsulate enzymes, preventing proteolytic inactivation and sedimentation in liquid detergents.

An insect-derived peptide targets ABA synthase to raise hormone levels without harmful chemical additives.

CRISPR base editing targets MHC Class I and II genes in hepatocytes, eliminating alloreactivity barriers that limit engraftment success.

Polydiallyldialkylammonium salt aggregates helper viruses for selective removal, reducing titers by 2-3 logs without affecting AAV yield.

Segmented affinity binding removes interfering substances from cell extract, preserving protein synthetic activity while reducing loss during purification.

Targeted ZmDA2 disruption via CRISPR systems boosts corn yield while reducing breeding cycle duration.

Engineered microbes convert plastic waste into terephthalic acid and ethylene glycol, avoiding mechanical property loss from conventional recycling.

Adeno-associated virus vectors deliver site-specific recombinases that assemble large nucleic acid segments into functional genes, overcoming packaging limits.

Dynamic temperature control resolves the contradiction between process simplicity and high productivity, increasing yield by up to 400%.

A CRISPR-Cas13a diagnostic system detects target nucleic acids using collateral RNA cleavage activity.

An enzyme cascade converts uridine monophosphate and D-galactose into UDP-galactose using a simplified reaction pathway.

Modified microorganisms increase S-adenosyl-L-homocysteine hydrolase activity to produce vanillin and vanillic acid from precursors in culture media.

Solyc10g038170 gene mutation induces parthenocarpy, eliminating labor-intensive hormone treatments and stabilizing yield under high temperature stress.

Trimmed Sanger traces and regression analysis deduce nucleic acid editing outcomes without massive parallel sequencing.

Hydrogel gradients concentrate ions to resolve the trade-off between detection sensitivity and speed, enabling 1000-fold amplification within minutes.

Nucleic acid modifications suppress multiple hormone pathways in teff, delivering lodging resistance without yield loss.

A CRISPR-Cas9 system uses programmable guide RNA to direct precise DNA cleavage for targeted genome modification.

Aligning assembled contigs against a pseudo-reference sequence to validate circular RNA junctions.

Engineered asparaginase variants withstand thermal stress during heating, reducing acrylamide formation while maintaining enzyme stability.

CRISPR systems cleave phage genomes for recombination while blocking wild-type replication to enrich recombinant strains.

A blocking sequence hybridizes with the guide RNA to form a cis-blocked stem, reducing off-target cleavage while maintaining high on-target activity.