Ion Analyzer Radical Temperature Control for Selective Dissociation
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Solution Overview
Problem
Current ion analysis methods, such as collision-induced dissociation, struggle to selectively dissociate ions at specific sites, and methods like electron transfer dissociation and electron capture dissociation are inefficient for analyzing monovalent positive ions, while the energy of radicals used for dissociation is difficult to control, leading to inadequate dissociation or unwanted dissociation at incorrect sites.
Innovation Solution
An ion analyzer that includes a reaction chamber, a radical irradiation unit, a standard substance supply unit, and a radical temperature calculation unit, allowing for the measurement of radical temperature by analyzing the amount of product ions generated from standard substances with known activation energies, enabling precise control of radical energy for effective ion dissociation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If collision-induced dissociation method is used to dissociate ions, then various ions can be dissociated, but the capability in selecting a position where ions are dissociated is poor
Solution Approach 1:
The patent changes the energy parameter of radicals by controlling radical temperature to achieve specific dissociation positions. By adjusting radical temperature, the invention enables selective dissociation at desired bond positions while maintaining versatile ion dissociation capability across different ion types.
2Measurement precision
If electron transfer dissociation or electron capture dissociation method is used to specifically dissociate peptides at amino acid linkages, then position-specific dissociation is achieved, but monovalent positive ions cannot be analyzed effectively
Solution Approach 1:
The patent changes the energy parameter of radicals by controlling radical temperature to achieve specific dissociation positions. By adjusting radical temperature, the invention enables selective dissociation at desired bond positions while maintaining versatile ion dissociation capability across different ion types.
3Measurement precision
If radicals with low temperature are used to irradiate precursor ions, then unwanted dissociation at incorrect sites is avoided, but dissociation efficiency is insufficient
Solution Approach 1:
The patent implements dynamic control of radical temperature to optimize both dissociation position accuracy and efficiency. By dynamically adjusting radical temperature based on analysis requirements, the system achieves high precision dissociation at specific positions while maintaining high dissociation efficiency through optimal energy matching between radicals and target bonds.
4Productivity
If radicals with excessively high temperature are used to irradiate precursor ions, then dissociation efficiency is improved, but dissociation occurs at undesired positions
Solution Approach 1:
The patent implements dynamic control of radical temperature to optimize both dissociation position accuracy and efficiency. By dynamically adjusting radical temperature based on analysis requirements, the system achieves high precision dissociation at specific positions while maintaining high dissociation efficiency through optimal energy matching between radicals and target bonds.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables precise dissociation of ions by measuring and controlling radical temperature, improving the efficiency of ion analysis and allowing for specific dissociation of peptides and other compounds, enhancing the accuracy of mass spectrometry and ion mobility analysis.
Implementation Method 1
activation energy of reaction in which the predetermined kind of radicals are added is known
Implementation Method 2
an ion measurement unit configured to measure an amount of predetermined product ions generated from precursor ions
Implementation Method 3
obtain a radical temperature based on a relationship between the amount of the radicals obtained for each of the plurality of kinds of standard substances and the activation energies
Data Source
AI summary
An ion analyzer includes a reaction chamber into which precursor ions derived from a sample component are introduced, a radical irradiation unit that generates and emits a predetermined type of radicals, a standard substance supply unit that individually supplies kinds of standard substances to the reaction chamber, where activation energy of radical addition reaction is known for each of the kinds of standard substances, and the activation energies are different in magnitude, an ion measurement unit that measures an amount of predetermined product ions generated from precursor ions derived from the standard substance by irradiation with the radicals, and a radical temperature calculation unit that obtains an amount of radicals that caused the radical addition reaction from the amount of the predetermined product ions and obtains a radical temperature based on a relationship between the amount of the radicals obtained for each kind of standard substance and activation energy.