Zero-Valent Iron Nanoparticles Enhance Microbial Lipid Yield

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Solution Overview

Problem

Current methods for enhancing the photosynthetic capacity, growth, and lipid production in microorganisms such as Fremyella diplosiphon do not effectively utilize nanotechnology, limiting their efficiency and productivity in bioreactors.

Innovation Solution

Complexing nano- and micro-particles, such as gold and zero-valent iron nanoparticles, with photosynthetic microorganisms to enhance growth rates and lipid production by improving light capture and scatter, and optimizing bioreactor conditions with specific light wavelengths and pulsations.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If conventional methods are used for enhancing photosynthetic capacity and growth in microorganisms, then basic growth is maintained, but photosynthetic efficiency and lipid production remain limited

Engineering Contradiction:
Improvephotosynthetic efficiency and lipid productionVSAvoidcomplexity of introducing nanotechnology
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent applies composite materials by combining nanoparticles (metal, ceramic, or carbon-based) with microorganisms to create a hybrid system. The nanoparticles are integrated into the microbial cells or their environment, forming a composite structure that enhances photosynthetic efficiency and lipid production while maintaining biological functionality.

Inventive Principle:
Principle #40Composite materials

Solution Approach 2:

The patent utilizes parameter changes by modifying physical and chemical properties of the system through nanoparticle introduction. This includes changing light absorption characteristics, surface area to volume ratio, and catalytic properties, which collectively improve photosynthetic capacity and metabolic output without fundamentally altering the microbial system.

Inventive Principle:
Principle #35Parameter changes

2Speed

If artificial light is used to enhance bioreactor growth rate, then growth speed increases, but energy costs and operational expenses increase

Engineering Contradiction:
Improvebioreactor growth rateVSAvoidartificial light energy consumption
Core Design Contradiction:
SpeedVSUse of energy by stationary object

Solution Approach 1:

The patent changes the optical parameters of the system by introducing nanoparticles with specific light-absorbing and light-scattering properties. These particles enhance natural light utilization efficiency, allowing the system to achieve higher growth rates using natural or reduced artificial light, thereby lowering energy consumption.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The nanoparticle-enhanced microorganisms exhibit improved self-capability to capture and utilize light energy. The nanoparticles act as natural light-harvesting agents that work autonomously with the microbial photosynthetic apparatus, reducing dependence on external artificial light sources and associated energy inputs.

Inventive Principle:
Principle #25Self-service

3Productivity

If nanoparticle complexing is implemented to enhance growth and lipid production, then productivity increases, but manufacturing complexity and process difficulty increase

Engineering Contradiction:
Improvegrowth rate and total lipid contentVSAvoidease of nanoparticle complexing process
Core Design Contradiction:
ProductivityVSEase of manufacture

Solution Approach 1:

The patent optimizes manufacturing ease by controlling nanoparticle parameters such as size, shape, surface charge, and material composition. By selecting appropriate parameter ranges, the patent enables spontaneous or facilitated complexing between nanoparticles and microorganisms, reducing process complexity while maintaining enhancement effects.

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Significant increases in growth rates and total lipid content are achieved, reducing the need for artificial light and lowering costs, while improving the quality and yield of biofuels and bioproducts.

Implementation Method 1

enhance growth rates and lipid production by improving light capture and scatter

Methodology Applied
Scientific EffectLight scattering: Scattering

Implementation Method 2

These organisms convert light energy into chemical energy through photosynthesis

Methodology Applied
Scientific EffectPhotosynthesis: Photosynthesis

Data Source

PatentUS20240352404A1Composition and method for enhancing photosynthetic efficiency, growth and/or lipid production of microorganisms
Publication Date: 2024.10.24 MORGAN STATE UNIVERSITY
  • US20240352404A1 patent drawing
  • US20240352404A1 patent drawing
  • US20240352404A1 patent drawing

AI summary

Compositions including metal nano-and/or micro-particles in solution with photosynthetic bioproduct producing microorganisms. These light harvesting complexes increase growth rates and photosynthetic efficiency of the constituent microorganisms, reducing the light required for a specific production level, or increases production for a specific light level. Compositions including zero-valent iron nanoparticles (nZVIs) in solution with photosynthetic bioproduct producing microorganisms. The nZVIs cause increased growth, lipid production and fatty acid production.