Bacteria TRIZ Cases

Deleting inhibitory prophages and activating Orf14 expression resolves productivity limits without increasing fermentation complexity.

A CRISPR-Cas9 genetic tool enables targeted homologous recombination in solventogenic Clostridium bacteria.

Homology arms guide targeted integration into the Spirulina genome, resolving random mutation trade-offs.

Nitrogen-containing polyethylene glycol-based nonionic surfactant sterilizes microbial cells while maintaining high enzyme activity levels.

Fructosamine deglycase converts amino acids and fructose-6-phosphate into glucosamine via enzymatic catalysis.

Tight ultrafiltration membranes recover nutrients from syngas fermentation broth to maintain continuous microbial activity.

Anaerobic fermentation with engineered enzymes eliminates oxygen risks and toxic catalysts, enabling safer butadiene production at mild temperatures.

A water-dissolvable device releases treatment agents through a dual-solubility core structure, reducing sewage clogging and replenishment frequency.

Recombinant Escherichia coli strain with modified tryptophan operon expression regulatory regions overcomes low production yields and anthranilate accumulation.

EchR mutations and vdh inactivation eliminate the lag period, preventing vanillin oxidation to vanillic acid.

Lactobacillus fermentum biofilm compositions block viral entry via ACE2 receptor binding, addressing the lack of effective coronavirus treatments.

Lactobacillus plantarum strain TAK 59 accelerates lactic acid production to resolve inadequate pH reduction in low dry matter forage, preserving nutrients.

Suspend lactic acid bacteria in nutrient-poor media to generate antimutagenic compounds, bypassing bacterial mortality in the digestive tract.

SSAP-SSB pairs replace Redβ to resolve low editing efficiency in non-E. coli species, enabling rapid multiplex genomic mutations.

Introducing zero-valent iron nanoparticles boosts microbial lipid yield by improving light scattering, reducing artificial energy consumption.

Disrupting multidrug efflux pumps in E. coli prevents substrate degradation, enabling higher yields of pharmaceutical compounds like vitamin D3.

Lytic bacteriophages target harmful gut bacteria to release nutrients that support beneficial microbial growth.

Novel nucleic acid molecules SPL1, SPL7, and SPL13 drive target gene expression in Corynebacterium microorganisms.

Abolishing Spo0A expression in thermophilic Moorella bacteria eliminates sporulation, reducing lag phase duration and enhancing CO2 fixation efficiency.

Engineered Escherichia coli assimilates sucrose via CscA hydrolase to produce DOI without expensive mannitol.

Human Hsp70 expression in E. coli triggers lac promoter transcription, eliminating IPTG toxicity and cost while maintaining high productivity.

Engineered E. coli strains achieve high succinate titers in simple mineral salt media by eliminating heterologous genes and complex fermentation requirements.

Modular genetic extraction reduces manufacturing complexity while maintaining genomic stability over 500 generations.

Monoclonal antibodies target the Hcp protein of the Type Six Secretion System, neutralizing bacterial virulence without inducing antibiotic resistance.

Engineered recombinant Escherichia coli utilizes mutated ldhA to synthesize high optical purity D-lactate.

A recombinant Corynebacterium strain engineered to produce putrescine by deleting the NCgl2523 transcriptional repressor gene.

Aerobic biomass granules remove phosphorus via controlled pH and alternating phases, eliminating metal salt pollution.

Applying pulsed electrical current to electro-autotrophic microbes stimulates resonant molecules within their electron transport chains.

Endogenous ethylamine synthesis eliminates volatile substrate handling, resolving safety hazards while maintaining high production efficiency.

Retaining fermentation broth as seed eliminates separate cultivators, lowering contamination risk and waste disposal while maintaining productivity.

Quadruply coated lactic acid bacteria improve intestinal mucosa attachment efficiency by nesting hyaluronic acid layers with porous particles.

Pseudomonas sp. strain TKU015 ferments aquatic waste powder to produce nattokinase, chitinase, and chitosanase enzymes.

Engineered microorganisms convert rebaudioside E to rebaudioside D4, resolving inconsistent natural extraction yields and high isolation costs.

A glucose permease library optimizes transport rates to uncouple flux from phosphoenolpyruvate utilization, reducing glycolytic by-products and improving yield.

Magnetic and fluorescent particles enable rapid antibiotic susceptibility testing by resolving the contradiction between speed and equipment complexity.

Carboxymethyl dextran microcarriers use negative charge and RGD sequences to resolve the trade-off between strong cell adhesion and gentle harvesting.

Symbiotic lactic acid microorganisms accelerate mineral matter synthesis in living structures, resolving slow biological production rates.

Lactobacillus plantarum strains reduce cholesterol absorption via bile salt hydrolase activity, addressing ineffective conventional probiotic formulations.

Engineered microbes replace energy-intensive petrochemical synthesis with biological fermentation to lower capital costs and reduce processing temperatures.

Deletions in aceA, ldhA, and pts genes redirect carbon flow to succinic acid, overcoming low yields from energy-intensive glucose uptake.

A 4-oxo-2-pentenoic acid composition activates Nrf2 and inhibits NF-kB to reduce oxidative stress.

Thermostable enzyme cocktails from Caldicellulosiruptor bescii degrade cellulose and hemicellulose into fermentable sugars.

Specific bacterial isolates restore intestinal microbiota diversity, resolving the contradiction between fecal transplant efficacy and patient acceptance.

Salt-tolerant lactic acid bacterium increases blood iron concentration without complex fermentation processes.

Inactivating the glg operons in Enterobacteriaceae bacteria extracts competing carbon sinks to increase L-amino acid accumulation.

Specific Lactobacillus plantarum strains resolve microbial imbalance by producing hydrogen peroxide and lactic acid to restore vaginal health.

Mutant Amycolatopsis strains convert ferulic acid to vanillin, reducing degradation to vanillic acid and increasing yield.