Antimutagenic Substance Production via Lactic Acid Bacteria Suspension
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Solution Overview
Problem
Current methods for administering lactic acid bacteria to prevent cancer are inefficient due to high mortality rates in the stomach and intestines, requiring extensive strain screening and genetic recombination techniques, and existing methods for producing antimutagenic substances from lactic acid bacteria are complex and costly, with unclear effectiveness.
Innovation Solution
Lactic acid bacteria are suspended in an oligotrophic or atrophic medium, such as physiological saline or phosphate buffered saline, for a medium to long period at low temperatures or briefly at high temperatures, or dried using freeze, heat, or reduced pressure drying methods, to generate antimutagenic substances effective against heterocyclic amines.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If lactic acid bacteria are administered to prevent cancer, then antimutagenic effects are achieved, but high mortality rates in stomach and intestines reduce effectiveness
Solution Approach 1:
The invention extracts and utilizes antimutagenic substances produced by lactic acid bacteria, separating this beneficial function from the bacterial cell itself. By using the culture medium containing these substances instead of administering live bacteria, the solution avoids bacterial mortality in the digestive tract while maintaining antimutagenic effectiveness.
Solution Approach 2:
The culture medium acts as an intermediary carrier of antimutagenic substances. Instead of directly administering bacteria that would die in the stomach and intestines, the invention uses the culture medium as a mediator to deliver the active antimutagenic components, bypassing the problem of bacterial mortality.
2Reliability
If extensive strain screening and genetic recombination techniques are used to improve bacterial survival, then antimutagenic properties are enhanced, but production complexity and cost increase
Solution Approach 1:
The invention extracts the desired antimutagenic function from the bacterial cell and transfers it to the culture medium. This eliminates the need for complex strain screening and genetic recombination techniques to improve bacterial survival, as the antimutagenic substances are stable in the medium without requiring live bacteria.
Solution Approach 2:
Instead of investing in expensive and complex genetic engineering to create long-lived bacteria, the invention uses a simpler approach: producing antimutagenic substances in a culture medium that can be administered without requiring the bacteria to survive. This replaces complex biological systems with a simpler chemical solution.
3Quantity of substance
If conventional extraction methods are used to obtain antimutagenic substances, then production is achieved, but process complexity and cost increase
Solution Approach 1:
The invention maintains antimutagenic substances in the culture medium through continuous production by lactic acid bacteria. Rather than using complex extraction processes to obtain these substances, the system allows continuous accumulation in the medium, simplifying the production process while maintaining high yields.
Solution Approach 2:
The lactic acid bacteria continuously produce and maintain antimutagenic substances in the culture medium without requiring external intervention or complex extraction processes. The system is self-sustaining, with bacteria naturally producing the substances that accumulate in the medium for direct use.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method allows for the simple and cost-effective mass production of antimutagenic substances with antioxidant properties, effectively neutralizing carcinogenic substances like heterocyclic amines, and demonstrating antimutagenic activity in both in vitro and in vivo tests.
Implementation Method 1
lactic acid bacteria generate antimutagenic substances... when live lactic acid bacterial cells are suspended in an oligotrophic or atrophic medium
Implementation Method 2
dried using a method from among a freeze drying method, a heat drying method, a reduced pressure drying method
Implementation Method 3
dried using a method from among a freeze drying method, a heat drying method, a reduced pressure drying method
Implementation Method 4
dried using a method from among a freeze drying method, a heat drying method, a reduced pressure drying method
Data Source
AI summary
Disclosed is a method for producing an antimutagenic substance by using a lactic acid bacteria, which can produce the antimutagenic substance in a large quantity in an extremely simple manner and is economically advantageous; particularly a method for producing an antimutagenic substance effective for a carcinogenic substance, particularly a heterocyclic amine (HCA) which is a carcinogenic substance derived from a food, by using a lactic acid bacteria. Specifically disclosed is a method for producing an antimutagenic substance by using a lactic acid bacteria, which is characterized by suspending the lactic acid bacteria in a poorly nutrient or nutrient-free medium and leaving the lactic acid bacteria in the medium. Preferably, the poorly nutrient or nutrient-free medium is selected from the group consisting of physiological saline, a phosphate-buffered saline without a calcium or magnesium salt (PBS (−)), a citrate buffer, a distilled water, an ion-exchanged water, a natural water, a well water, a tap water, a mineral-added water, a vitamin-added water, an ion supply beverage for an athlete and the like. More preferably, the lactic acid bacteria is Lactobacillus plantarum strain KK-2503 or Lactobacillus alimentarius strain KN-15.