Lactobacillus plantarum TAK 59 Silage Additive
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Solution Overview
Problem
The challenge in precision livestock farming is to ensure high-quality feed production through silage fermentation, particularly for materials with low dry matter content or high buffering capacity, where natural fermentation is difficult to control and results in poor preservation of nutrients due to inadequate pH reduction.
Innovation Solution
The use of Lactobacillus plantarum strain TAK 59 NCIMB42150 as a microbiological silage additive, which inoculates into the ensiled forage, accelerating lactic acid fermentation and reducing pH, thereby inhibiting undesirable microorganisms and enhancing nutrient preservation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If natural fermentation is used for plant material with low dry matter content or high buffering capacity, then the process is simpler, but the pH reduction is inadequate and nutrient preservation is poor
Solution Approach 1:
A specific Lactobacillus plantarum strain (TAK 59 NCIMB 42150) is introduced as a microbial intermediary to mediate the fermentation process. This strain acts as a catalyst that accelerates lactic acid production and pH reduction in difficult-to-ensile materials, resolving the contradiction between process simplicity and fermentation reliability.
Solution Approach 2:
The invention changes the microbial parameter by selecting a specific Lactobacillus plantarum strain with optimized characteristics for rapid lactic acid production. This parameter change enables effective pH reduction in materials with low dry matter content or high buffering capacity, improving reliability without complicating the manufacturing process.
2Reliability
If chemical additives are used to improve fermentation of difficult materials, then pH reduction is more effective, but the feed quality and safety may be compromised
Solution Approach 1:
The patent uses a specific Lactobacillus plantarum strain as a biological intermediary to achieve pH reduction, replacing chemical additives. This microbial mediator naturally produces lactic acid through fermentation, achieving effective pH control while maintaining feed safety and quality without introducing harmful chemical residues.
Solution Approach 2:
The invention substitutes chemical mechanisms with biological mechanisms. Instead of using chemical acids and additives to reduce pH, the system employs a specifically selected Lactobacillus plantarum strain that biologically produces lactic acid, replacing potentially harmful chemical interventions with a safe biological process.
3Loss of substance
If fermentation time is extended to improve nutrient preservation, then more nutrients are preserved, but the process becomes less productive
Solution Approach 1:
The patent optimizes the microbial parameter by selecting a Lactobacillus plantarum strain with rapid growth and high lactic acid production rates. This parameter optimization enables the system to achieve effective nutrient preservation in a shorter time frame, resolving the contradiction between nutrient preservation and fermentation speed.
Solution Approach 2:
The selected Lactobacillus plantarum strain is inoculated at an optimized concentration to initiate rapid fermentation. This preliminary action of pre-selecting and pre-inoculating with an optimized strain creates a head start in the fermentation process, enabling faster pH reduction and nutrient preservation without requiring extended fermentation times.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach improves the fermentation quality of silage by increasing lactic acid production, reducing ethanol, acetic acid, and ammonia nitrogen levels, leading to better preservation and reduced nutrient loss across various ensiling difficulties, maintaining feed quality in an anaerobic condition for extended periods.
Implementation Method 1
ensiling is considered to be lactic acid fermentation with no oxygen... in anaerobic conditions, micro-organisms (ideally lactic bacteria) ferment water-soluble carbohydrates into organic acids
Implementation Method 2
The antimicrobial compounds produced by Lactobacillus plantarum TAK 59 NCIMB42150 inhibited the decrease of plant-origin clostridia by 19.81 per cent
Data Source
AI summary
The invention provides the isolated microorganism Lactobacillus plantarum TAK 59 NCIMB42150, which is used for accelerating the fermentation of feed, for increasing the concentration of lactic acid and for reducing pH, hence decreasing the loss of nutrients in feed. L. plantarum TAK 59 decreasess the concentration of ammonia nitrogen and butyric acid in feed. Usage of the microorganism in ensiling suppresses the function of proteolytic and pathogenic microorganisms (enteropathogens and Clostridium) in feed. Lactobacillus plantarum TAK 59 can be used as silage additive in easy and moderately difficult to ensile forage and for prolonging the storage life of feed.