Lactobacillus paracasei Strain CNCM I-3689 Antimicrobial Viability
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Solution Overview
Problem
Current probiotic strains of Lactobacillus paracasei subsp. paracasei do not consistently exhibit antimicrobial and immunomodulatory properties, and their viability in dairy products and intestinal environment is a concern, limiting their effectiveness as probiotics.
Innovation Solution
A novel strain of Lactobacillus paracasei subsp. paracasei (CNCM I-3689) with specific characteristics, including a single CRISPR locus and ability to inhibit pathogenic microorganisms and exhibit anti-inflammatory properties, is isolated and developed, along with methods for mutagenesis and genetic transformation to enhance its properties.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional probiotic strains are used, then basic probiotic function is provided, but antimicrobial and immunomodulatory properties are inconsistent
Solution Approach 1:
The patent applies parameter changes by selecting and characterizing specific strains with defined genetic markers (CRISPR-Cas system configuration, specific gene presence/absence) to achieve consistent antimicrobial and immunomodulatory properties. The invention establishes specific parameter thresholds for strain selection and uses mutagenesis/genetic transformation to modify parameters when needed.
Solution Approach 2:
The patent creates replicable strain models with standardized genetic characteristics that can be consistently produced and tested. By establishing specific genetic markers and phenotypic criteria, the invention enables copying of successful strain configurations across multiple batches and applications, ensuring reliability.
2Reliability
If probiotic strains are incorporated into dairy products, then probiotic delivery is achieved, but viability during production and storage is compromised
Solution Approach 1:
The patent applies preliminary action by pre-adapting strains to dairy product conditions through controlled fermentation processes before final product formation. The strains are pre-conditioned to withstand production and storage environments, establishing robust viability prior to the stress of prolonged storage.
Solution Approach 2:
The invention modifies environmental parameters during production (temperature, pH, oxygen levels) to optimize strain survival. Specific fermentation conditions are established that enhance strain hardiness and protect viability throughout the product shelf life.
3Reliability
If strain characteristics are optimized for antimicrobial activity, then pathogen inhibition is improved, but growth on milk may be affected
Solution Approach 1:
The patent applies local quality by enabling the strain to exhibit different functional properties in different environments: strong antimicrobial activity against pathogens in the intestinal environment, while maintaining good growth characteristics on milk during fermentation. The strain's metabolic pathways are optimized to switch between growth mode and antimicrobial mode based on environmental conditions.
Data Source
AI summary
The invention relates to a novel strain of Lactobacillus paracasei subspecies paracasei, having antimicrobial and immunomodulatory properties, and to compositions containing said strain.