Lateral Flow Test Strip for oxLDL/β2GPI Complex Detection
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Solution Overview
Problem
Current methods for detecting the oxLDL/β2GPI complex, such as ELISA, require high experimental skill and are not suitable for high-throughput analysis due to laborious procedures and the need for repeated reagent additions and washing steps.
Innovation Solution
A lateral flow assay using a test strip with a first binding component that specifically binds to the oxLDL/β2GPI complex, such as an anti-β2GPI antibody, and a second binding component that labels the captured complex, enabling quicker and more sensitive detection without requiring extensive technical expertise.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If ELISA method is used to detect oxLDL/β2GPI complex, then detection sensitivity can be achieved, but the procedure becomes laborious and requires high experimental skill
Solution Approach 1:
The patent replaces the manual mechanical operations of ELISA (repeated reagent additions, incubations, and washings) with a lateral flow assay system that uses capillary action and pre-assembled test strips. This substitution eliminates the need for skilled manual manipulation while maintaining detection sensitivity through the use of specific antibodies and labeled detection complexes.
Solution Approach 2:
The lateral flow test strip is designed to perform the detection function autonomously without requiring operator intervention for each step. The sample automatically flows through the strip via capillary action, reagents are pre-loaded, and the detection reaction occurs automatically, making the system self-sufficient and eliminating the need for high experimental skill.
2Measurement precision
If ELISA method is used to detect oxLDL/β2GPI complex, then detection can be performed, but it is not suitable for high-throughput analysis due to laborious procedures
Solution Approach 1:
The detection function is segmented into independent lateral flow test strips, each capable of autonomous operation. This segmentation allows multiple samples to be processed simultaneously using identical simple procedures, enabling high-throughput analysis without the laborious repeated steps required by ELISA for each sample.
Solution Approach 2:
The lateral flow assay skips the time-consuming intermediate steps of ELISA (multiple washings, reagent additions, and incubations) by using a continuous flow system where the sample moves rapidly through predetermined zones. This rushing through of detection steps maintains detection capability while dramatically increasing processing speed and throughput.
3Measurement precision
If conventional detection methods are used, then detection can be performed, but the procedure is time-consuming and complex
Solution Approach 1:
The test strip is pre-assembled with all necessary components (capture antibodies, detection antibodies, labels, and control elements) in their correct positions before use. This preliminary preparation eliminates the need for time-consuming setup and procedural steps during actual detection, allowing rapid and accurate results while maintaining detection accuracy through pre-optimized reagent ratios and positioning.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method allows for easier and faster detection of the oxLDL/β2GPI complex with a broader dynamic range compared to conventional ELISA methods, facilitating quicker and more accurate monitoring of arteriosclerosis progression without the need for professional skill.
Implementation Method 1
a step of capturing the oxLDL/β2GPI complex in the test sample in a predetermined position on the test strip by a first binding component that binds to the oxLDL/β2GPI complex
Implementation Method 2
a step of labeling the oxLDL/β2GPI complex captured in the predetermined position on the test strip by making a second binding component comprising a labeling agent be bound to the captured oxLDL/β2GPI complex
Implementation Method 3
uses a test strip for lateral flow assay
Data Source
AI summary
The object of the present invention is to provide an easy and quick detection method for detecting an oxLDL/β2GPI complex in biological samples, and a detection kit therefor. The present invention attains this object by providing a detection method for detecting an oxLDL/β2GPI complex, which uses a test strip for lateral flow assay, comprising a step of capturing the oxLDL/β2GPI complex in the test sample in a predetermined position on the test strip by a first binding component that binds to the oxLDL/β2GPI complex; and a step of labeling the oxLDL/β2GPI complex captured in the predetermined position on the test strip by making a second binding component comprising a labeling agent be bound to the captured oxLDL/β2GPI complex, and a detection kit therefor.