LED Light Irradiation for Comet-Assay Cancer Detection
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing cancer diagnosis methods are complex, time-consuming, and costly, requiring multiple medical examinations that consume significant resources.
Innovation Solution
A light irradiation apparatus that cuts genetic material into sections of different sizes and deforms it to varying degrees, using LEDs to emit light in specific wavelength bands to distinguish between normal and abnormal samples based on cytotoxicity and genotoxicity, with a Comet assay for analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If multiple medical examinations (angiography, CT, MRI, endoscope, immunological examination, cell examination, pathological examination) are performed to diagnose cancer, then diagnostic accuracy is improved, but examination time and cost increase significantly
Solution Approach 1:
The invention extracts and focuses on a single critical parameter (comet tail length) from the complex cancer diagnosis process. By using flow cytometry to measure only the comet tail length of irradiated cells, the method isolates the most informative feature that distinguishes cancerous from normal cells, eliminating the need for multiple comprehensive examinations while maintaining diagnostic accuracy
Solution Approach 2:
The invention changes the measurement parameter from traditional anatomical or biochemical markers to a physical parameter (comet tail length) that directly reflects genetic damage. This parameter change enables rapid, objective differentiation between normal and cancerous cells through flow cytometry, dramatically reducing examination time while preserving diagnostic precision
2Measurement precision
If multiple medical examinations are performed to diagnose cancer, then diagnostic accuracy is improved, but examination cost increases significantly
Solution Approach 1:
The invention extracts the essential diagnostic information from complex medical examinations and concentrates it into a single flow cytometry measurement of comet tail length. This extraction eliminates the need for expensive multi-modality imaging and multiple specialized tests, reducing examination cost while maintaining the ability to accurately identify cancerous cells
Solution Approach 2:
The invention uses a disposable, low-cost flow cytometry assay that can be performed on small cell samples. This inexpensive, single-use testing method replaces expensive, complex medical imaging and multiple specialized examinations, providing cost-effective cancer diagnosis without sacrificing accuracy
3Difficulty of detecting and measuring
If light is irradiated to cut genetic material into sections and create deformation for cancer diagnosis, then diagnostic capability is improved, but sample damage from external factors increases
Solution Approach 1:
The invention applies a controlled, partial action of light irradiation that is sufficient to induce measurable genetic damage (comet tail formation) but not so excessive as to cause complete cell death or severe sample degradation. This optimized irradiation level maintains diagnostic capability while minimizing harmful effects on the sample
Solution Approach 2:
The invention replaces mechanical or chemical sample preparation methods with optical irradiation that directly induces genetic damage. This substitution eliminates the need for complex mechanical tissue processing and chemical staining procedures that can damage samples, using light-induced comet assay instead
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate and cost-effective cancer diagnosis by simply providing a genetic material sample, reducing the time required for diagnosis and minimizing sample damage from external factors.
Implementation Method 1
the light may be transmitted into the sample to stimulate a photosensitive material in the sample, and thus, provided in a wavelength band in which reactive oxygen species are generated
Data Source
Figure 1
Figure 2~3
Figure 4a
AI summary
A light irradiation apparatus is an apparatus for analyzing a sample by applying light to the sample. The light cuts genetic material in a sample into fragments of mutually different sizes in accordance with a normal sample or an abnormal sample, modifies same to mutually different degrees, and determines whether the sample is normal or abnormal according to the degree of modification of the genetic material, wherein the irradiation amount or intensity of the light is provided so as to be an intensity by which the cytotoxicity level of the sample becomes equal to or greater than a predetermined level, and the ratio of the degree of modification of the genetic material of the normal sample to the degree of modification of the genetic material of the abnormal sample is set to be the minimum value outside an error range.