Lipase-Driven Alginate Hydrogel Preparation

The patent applies preliminary action by pre-mixing the alginate solution with the spermatozoa suspension before adding the GDL solution. This allows all other components to be prepared and positioned in advance, so that when GDL is added, the gelling process can proceed immediately without delay. The preliminary preparation of the alginate-spermatozoa mixture ensures that the actual gelling time is maximized while maintaining efficient process flow.

The patent applies segmentation by dividing the gelling process into distinct stages: (1) preparation of alginate solution, (2) addition of spermatozoa suspension, (3) pre-mixing period, and (4) final addition of GDL solution. This segmentation allows each step to be optimized independently - the pre-mixing stage ensures proper integration of components before gelling begins, while the separate GDL addition step provides reliable initiation of gel formation. The segmented approach transforms a single rushed operation into a controlled sequence of steps.

The patent applies preliminary action by completing the filling of spermatozoa into the alginate solution before initiating gelling with GDL. All filling operations are performed during the pre-mixing stage when the solution remains fluid and easy to handle. Once filling is complete, GDL is added and the solution immediately begins to gel, eliminating the need to rush the filling process during viscosity increase. This sequencing of operations resolves the contradiction between allowing gel structure formation and maintaining productivity.