Lipase Polypeptides for Enhanced Lipid Hydrolysis

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Solution Overview

Problem

Current technologies lack effective methods for producing polypeptides with specific lipase activity and efficient methods for their production and application, particularly in cleaning surfaces and hydrolyzing lipids.

Innovation Solution

Development of isolated polypeptides with specific sequence identities and variants, encoded by polynucleotides, which are used in recombinant expression vectors and host cells to produce polypeptides with enhanced lipase activity for applications in cleaning and lipid hydrolysis.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If current technologies are used for producing polypeptides with lipase activity, then production methods exist, but they lack effectiveness and efficiency in producing polypeptides with specific lipase activity

Engineering Contradiction:

Improvelipase activity effectivenessVSAvoidproduction efficiency

Core Design Contradiction:

ReliabilityVSProductivity

Solution Approach 1:

The patent applies parameter changes by identifying specific amino acid residues (positions 31, 139, 174, 206, 248) that can be mutated to enhance lipase activity. By systematically varying these parameters (amino acid sequences) and selecting variants with improved catalytic efficiency, the invention resolves the contradiction between reliability of lipase activity and productivity of production efficiency.

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent employs feedback mechanisms through high-throughput screening methods that measure lipase activity of variant polypeptides and feed this information back into the selection process. This allows iterative optimization where variants with higher lipase activity are identified and further refined, simultaneously improving both the reliability of lipase activity and the efficiency of the production process.

Inventive Principle:

Principle #23Feedback

2Productivity

If existing methods are used for cleaning surfaces and hydrolyzing lipids, then basic functionality is provided, but efficiency and effectiveness are limited

Engineering Contradiction:

Improvecleaning and hydrolysis efficiencyVSAvoidmethod effectiveness

Core Design Contradiction:

ProductivityVSReliability

Solution Approach 1:

The patent changes the parameters of the cleaning and hydrolysis methods by using engineered polypeptides with optimized amino acid sequences. These variants exhibit enhanced lipase activity, which directly improves the efficiency of lipid hydrolysis and surface cleaning applications while maintaining reliability through systematic validation of catalytic function.

Inventive Principle:

Principle #35Parameter changes

3Reliability

If polypeptides with high lipase activity are produced, then cleaning and hydrolysis applications are improved, but production methods need to be developed

Engineering Contradiction:

Improvelipase activityVSAvoidproduction method availability

Core Design Contradiction:

ReliabilityVSEase of manufacture

Solution Approach 1:

The patent segments the production process into distinct modules: (1) gene synthesis with specific coding sequences for variant polypeptides, (2) expression in suitable host systems, (3) purification protocols, and (4) application formulations. This segmentation makes the manufacture of high-activity lipase polypeptides systematically achievable and scalable.

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The patent performs preliminary action by designing and synthesizing optimized gene sequences before expression, and by establishing validated production protocols in advance. This preparatory work ensures that when production is undertaken, the methods are already optimized and reliable, making the manufacture of high-activity polypeptides straightforward.

Inventive Principle:

Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The solution enables the production of polypeptides with high lipase activity, facilitating efficient surface cleaning and lipid hydrolysis, addressing the limitations of existing technologies in this area.

Implementation Method 1

isolated polynucleotides encoding the polypeptides of the present invention

Methodology Applied

Scientific EffectGenetic encoding:

Implementation Method 2

polypeptides having lipase activity

Methodology Applied

Scientific EffectEnzyme catalysis: Enzyme

Implementation Method 3

methods of hydrolyzing a lipid

Methodology Applied

Scientific EffectHydrolysis: Hydrolysis

Data Source

PatentUS10246692B2Polypeptides having lipase activity and polynucleotides encoding same

Publication Date: 2019.04.02 NOVOZYMES AS

AI summary

The present invention relates to isolated polypeptides having lipase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.