Hydrolases TRIZ Cases

Mutated polypeptides resolve production efficiency trade-offs by enabling reliable lipid hydrolysis and surface cleaning.

Engineered recombinant microorganisms channel carbon flux through the mevalonate pathway to produce isoprenoids, eliminating toxic solvent extraction.

CRISPR/Cas9 editing of HLA genes increases donor cell immunocompatibility, reducing immune rejection risks in allogeneic transplantation.

Engineered bacteria convert renewable carbon sources into alpha-omega bifunctional fatty acids using specific enzyme pathways.

Engineered effector proteins and guide nucleic acids enhance target recognition specificity while maintaining high modification efficiency.

Targeted AdoMetase expression reduces lignin methylation, enhancing enzymatic saccharification efficiency for biofuel production.

A CRISPR-Cas9 complex with a supercharged protein and ssODN targets blood cell lineage genomes.

Novel nuclease proteins degrade contaminating DNA in fermentation broths, eliminating viscosity issues and regulatory risks while maintaining high productivity.

Demethylase enzymes in engineered cells convert opioids to safer analgesics, reducing addiction risk and production costs.

CRISPR enzymes cleave linear bacteriophage genomes for recombination, while exonucleases enrich circular intermediates to resolve low yield bottlenecks.

A wheat haploid inducer plant uses NLD gene inhibition to produce haploid progeny for breeding.

Minimal Type III-A Csm complex achieves site-specific RNA knockdown in vertebrates, resolving reconstitution complexity and organism applicability limits.

Nucleic acids enhance haploid induction rates in maize by targeting energy metabolism, bypassing time-consuming multi-generational selfing.

Zinc-binding motif suppresses premature cleavage during expression, boosting target protein yield and purity in mammalian cell culture.

Engineered aminoacyl-tRNA synthetase charges non-natural amino acids with high specificity for protein incorporation.

A 5'-phosphodiesterase converts recovered yeast cell wall RNA into 5'-ribonucleotides through enzymatic hydrolysis.

Ndrg2 knockout dendritic cells in a hydrogel scaffold promote angiogenesis and resolve chronic wound healing delays.

Targeted mutations in the IPT7 gene bypass complex transgenic limitations to deliver consistent yield improvements.

An enzyme additive degrades fumonisins into non-toxic substances without requiring molecular oxygen.

A recombinant microorganism expresses lipase under strong promoters to boost biosurfactant yields.

The lva operon converts levulinic acid into 3HV-CoA, resolving the unknown enzymatic pathway bottleneck.

Eukaryotic cells with elevated phosphatidic acid levels expand the endoplasmic reticulum membrane surface area to support higher protein production.

CRISPR/Cas9 edits the Ty-5 gene to create stable male sterile germplasm, solving unreliable natural fertility and lengthy breeding cycles.

Dimethyl ether extracts butanol from dilute aqueous solutions, bypassing energy-intensive distillation steps.

Phage-encoded Acr proteins inhibit Cas9 to reduce off-target effects while maintaining high gene editing productivity.

Nucleotide sequences encoding thioesterase enzymes catalyze efficient thioester formation.

Specific safe harbor loci on chromosomes 10, 11, 15, and 16 allow precise CRISPR-Cas9 insertion to resolve random integration risks.

Engineered autoprotease N pro derivative cleaves fusion polypeptides to yield homogeneous N-termini.

Deleting specific protease genes from filamentous fungal cells eliminates protein degradation, boosting full-length antibody yields.

Fungal deoxyribonuclease disrupts biofilms in laundry detergents, resolving bacterial enzyme instability while maintaining fabric whiteness.

Lipid nanoparticles encapsulate CasX:gNA complexes to bypass viral immune responses while maintaining high cellular uptake efficiency for HTT gene correction.