Lipase Variant Detergent Composition Odor and Stability
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Solution Overview
Problem
Current detergent compositions with lipase enzymes face challenges in maintaining stability and reducing odor generation, particularly when exposed to varying temperatures or incompatible components, leading to reduced efficacy and shorter shelf life.
Innovation Solution
A detergent composition featuring a lipase variant with specific substitutions, such as at positions 40, 118, and 244, and additional positions like 23, 51, 56, 231, and 233, which is encapsulated in a microcapsule formed by cross-linking a polybranched polyamine, enhancing stability and reducing odor generation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If wild-type Thermomyces lanuginosus lipase is used in detergent compositions, then good wash performance is achieved through hydrolysis of triglycerides, but odor generation increases and storage stability decreases
Solution Approach 1:
The patent applies parameter changes by modifying the amino acid sequence of the lipase enzyme through site-directed mutagenesis. Specific amino acid residues are substituted to alter the enzyme's catalytic properties, reducing its ability to generate short-chain fatty acids that cause odor while maintaining triglyceride hydrolysis activity for stain removal.
Solution Approach 2:
The patent applies local quality by making targeted modifications at specific locations (amino acid positions) within the lipase molecule. Rather than changing the entire enzyme structure, only particular catalytic sites are modified to achieve selective reduction of odor-causing byproducts while preserving overall enzymatic function.
2Productivity
If wild-type Thermomyces lanuginosus lipase is used in detergent compositions, then good wash performance is achieved, but storage stability and shelf life are reduced
Solution Approach 1:
The patent applies parameter changes by modifying the amino acid sequence of the lipase enzyme through site-directed mutagenesis. Specific amino acid residues are substituted to alter the enzyme's catalytic properties, reducing its ability to generate short-chain fatty acids that cause odor while maintaining triglyceride hydrolysis activity for stain removal.
Solution Approach 2:
The patent applies local quality by making targeted modifications at specific locations (amino acid positions) within the lipase molecule. Rather than changing the entire enzyme structure, only particular catalytic sites are modified to achieve selective reduction of odor-causing byproducts while preserving overall enzymatic function.
3Productivity
If lipase is exposed to significantly higher or lower temperatures than ambient temperature, then wash performance may be maintained, but enzyme activity and stability are reduced
Solution Approach 1:
The patent applies parameter changes by modifying the amino acid sequence of the lipase enzyme through site-directed mutagenesis. Specific amino acid residues are substituted to alter the enzyme's catalytic properties, reducing its ability to generate short-chain fatty acids that cause odor while maintaining triglyceride hydrolysis activity for stain removal.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The modified lipase variant composition demonstrates improved wash performance, reduced odor generation, extended shelf life, and increased thermostability, maintaining activity across different conditions.
Implementation Method 1
Lipases are important biocatalysts which have shown to be very useful in detergent compositions contributing to the removal of oily soils and stains by hydrolyzing triglycerides to generate fatty acids
Implementation Method 2
encapsulated in a microcapsule formed by cross-linking a polybranched polyamine
Data Source
AI summary
Detergent compositions that include a lipase variant of a parent lipase which has lipase activity and includes one or more substitutions corresponding to G23S, D27N, A40, F51I,L, E56R, D57N, V60E,K, K98I, N101D, R118, G163S, T231R, N233R, Y220F, T244E, and P256T using SEQ ID NO: 2 for numbering. Water-soluble unit dose articles including water-soluble film and a detergent composition including lipase variants. Methods of cleaning and/or treatment of surfaces using such compositions.