Hydrolases TRIZ Cases

Replacing petrochemical routes with recombinant microbes reduces environmental harm while maintaining industrial production efficiency.

Tissue-specific promoters restrict CRISPR activity to specific organs, preventing pleiotropic effects and controlling transgenic trait transmission.

Replacing customized proteins with programmable RNA guides reduces system complexity while maintaining high targeting specificity.

Encapsulated lipase variants reduce odor generation and extend shelf life while maintaining wash performance.

Graphene biosensors detect target nucleic acids via immobilized ribonucleoprotein complexes, eliminating PCR amplification to simplify instrumentation.

Novel CoA-dependent butyraldehyde pathways bypass fatty acid synthesis limits to boost propane yield despite poor ADO activity with butyraldehyde.

Segmenting targeting and cutting functions reduces system complexity while maintaining high precision in eukaryotic genome engineering.

Expand tumor infiltrating lymphocytes using a one-step process with TCR and CD28 agonists.

Novel DNase variants with specific amino acid substitutions enhance enzyme stability in detergent compositions.

A CRISPR-Cas9 nickase complex paired with a single-stranded donor oligonucleotide enables precise genomic mutation correction.

Chemically modified single-stranded DNA oligonucleotides serve as HDR donor templates to resist targeted nuclease activity.

Enzymatic hydrolysis of paprika oleoresin followed by medium-pressure column chromatography isolates beta-carotene from complex pigment mixtures.

Amino acid substitutions create thermostable phytase variants that maintain catalytic activity during demanding industrial operations.

CRISPR/Cas9 inactivation of endogenous retrovirus loci reduces viral contamination while maintaining recombinant protein productivity.

A test strip integrates dried enzyme and substrate pads connected by a porous membrane to generate a measurable signal upon sample contact.

Replacing erythromycin gene clusters with spinosad and rhamnose clusters in Saccharopolyspora erythraea resolves low yield and genetic instability.

Segmented barcoded agents resolve experimental complexity by mapping non-linear cellular circuit interactions through single-cell RNA sequencing.

Segmented enzymatic conversion of dihydroxyacetone phosphate and glyceraldehyde-3-phosphate produces fructose-6-phosphate, bypassing glucose inhibition.

Cloning S-RNase genes from self-compatible potatoes creates diploid inbred lines without wild gene linkage drag, accelerating breeding cycles.

Modified KZ144 endolysin polypeptides enhance thermal stability through targeted amino acid substitutions.

Glutaraldehyde pretreatment protects nitrilase from inactivation by aldehydes and hydrogen cyanide during glycolonitrile hydrolysis.

A sequential genetic modification method introduces DNA breaks with repair intervals between rounds.

Engineered polypeptides retain protease activity in acidic stomach environments and after steam treatment, improving protein digestibility.

Covalently closed dumbbell-shaped vectors extend transgene expression duration in primary cells while eliminating restriction endonuclease costs.

Precise amino acid substitution in the ANP32A gene via CRISPR/Cas9 induces avian influenza resistance while preserving normal cellular functions.

Acid-stable esterases degrade polyesters at pH 3 to 6, eliminating base addition and salt production during depolymerization.

Isothermal RPA amplification with Cas13a cleavage enables rapid one-hour detection of kidney allograft rejection biomarkers in urine.

Combining DGA1 and DGA2 expression with TGL3 knockout resolves unpredictable lipid production consistency by preventing degradation.

Amide-functionalized ordered mesoporous carbon chemically conjugates degradative enzymes to its surface for enhanced stability.

Programmed guide RNAs and mutated Cas9 variants resolve the trade-off between targeting versatility and specificity, reducing toxicity in eukaryotic cells.

Engineered activatable Cas9 proteins enable precise spatial and temporal control of gene editing through site-specific caged lysine residues.

CRISPR/Cas9 targets the SELF PRUNING gene in peanut to alter plant architecture and induce determinate growth habits.

Site-directed mutagenesis of Acidovorax facilis nitrilase boosts reaction rates while maintaining enzyme lifespan at elevated temperatures.

Combining a large targeting vector with CRISPR Cas9 resolves the trade-off between precision and efficiency for targeted genetic modification in rat cells.

A single-bath enzymatic method converts plant-derived biomass into high-quality textile-grade fibers through sequential treatments in one water bath.

Replacing complex protein-DNA recognition with RNA-mediated copying, the C2c2 system resolves scaling contradictions in eukaryotic genome engineering.

A modified host cell produces isopentenol via phosphomevalonate decarboxylase and a specific phosphatase.

VbAgo uses single-stranded guide DNA to target RNA, avoiding collateral DNA damage and reducing synthesis costs.

A sparse additive mechanism shift variational autoencoder models cellular responses using disentangled latent representations.

Extracting and overexpressing a specific FatB thiosterase enzyme replaces complex multi-gene modifications to boost C8-C12 fatty acid production.

Autocatalytic RNA sensor converts trigger hybridization into translational output, reducing delivery complexity by leveraging endogenous ADAR levels.

Engineered yeast resolves NADPH supply bottlenecks by integrating pentose phosphate and malic enzyme pathways for efficient ectoine synthesis.

Direct Cas13 detection of aberrant viral RNA avoids amplification errors from RT-PCR, ensuring accurate quantification of target sequences.

A dCas9-Clo051 fusion protein enables targeted nucleic acid insertion into induced pluripotent stem cells.

R372A and K375A mutations disable integration to stop unwanted genetic changes during transposon excision from iPSCs.

CRISPR-mediated CD5 modification allows engineered cells to resist immunotherapeutic agents, preventing on-target off-disease cytotoxicity.

Engineered Type V-I CRISPR-Cas systems use novel RNA guides and effector proteins to target nucleic acids.