MALDI Matrix Transfer for Precise Multi-Matrix Sample Preparation

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Solution Overview

Problem

Existing MALDI mass spectrometry methods are limited by the inability to apply multiple optimal matrices to a single sample, leading to uneven crystal diameters and reduced sensitivity and accuracy due to operator-dependent processes.

Innovation Solution

A method and device that apply a laser beam to a matrix-containing base to make the matrix fly and position multiple kinds of matrices at predetermined locations on the sample, using an optical vortex laser beam to enhance precision and reduce scattering.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If a single matrix is applied to a sample using conventional methods, then the sample can be analyzed, but multiple optimal matrices cannot be applied to the same sample, limiting analysis sensitivity and accuracy

Engineering Contradiction:

Improveability to apply multiple matricesVSAvoidanalysis sensitivity and accuracy

Core Design Contradiction:

Adaptability or versatilityVSMeasurement precision

Solution Approach 1:

The patent divides the matrix application process into multiple discrete steps, where different matrices are applied to different regions of the sample in sequence. Each matrix is deposited as separate microcrystals at predetermined positions, allowing multiple matrices to be used on a single sample without mixing or interference.

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The patent applies different matrices to different local regions of the sample based on the specific analytical requirements for each analyte. Each matrix is positioned at predetermined locations where it can optimally analyze specific target molecules, creating locally optimized conditions across the sample.

Inventive Principle:

Principle #3Local quality

2Ease of manufacture

If operator-dependent methods are used to apply matrices, then the process is simple, but uneven crystal diameters result, reducing reproducibility

Engineering Contradiction:

Improveprocess simplicityVSAvoidcrystal diameter uniformity

Core Design Contradiction:

Ease of manufactureVSManufacturing precision

Solution Approach 1:

The patent replaces manual operator-dependent matrix application with an automated laser-based system. The laser beam automatically deposits matrices as uniform microcrystals at predetermined positions, eliminating the variability introduced by manual spraying or spotting techniques while maintaining process simplicity through automation.

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent uses laser parameters (wavelength, pulse duration, intensity) to control the matrix deposition process precisely. By adjusting these parameters, uniform microcrystals of controlled diameter are formed consistently, regardless of operator skill or manual technique variations.

Inventive Principle:

Principle #35Parameter changes

3Ease of operation

If matrices are applied using conventional spraying or spotting, then the application is straightforward, but scattering occurs and precision positioning is difficult

Engineering Contradiction:

Improvematrix application easeVSAvoidpositioning accuracy

Core Design Contradiction:

Ease of operationVSMeasurement precision

Solution Approach 1:

The patent replaces mechanical spraying or spotting mechanisms with a laser-based optical system. The laser beam can be precisely positioned and focused to deposit matrices at exact predetermined locations without the scattering inherent in spray methods or the manual positioning errors of spotting techniques.

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent transitions from two-dimensional spray deposition to highly localized three-dimensional microcrystal formation at precise coordinates. The laser enables control over the vertical dimension of crystal growth while maintaining precise horizontal positioning, eliminating scattering and achieving accurate placement.

Inventive Principle:

Principle #17Another dimension (Dimensionality change)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Enables high-sensitivity imaging mass spectrometry by allowing multiple matrices to be applied accurately to a single sample, improving analysis sensitivity and reproducibility.

Implementation Method 1

applying a laser beam to a base containing a powder of a dried and crystallized matrix disposed on a surface of the base, wherein the laser beam is applied to a surface of the base opposite to the surface on which the matrix is disposed, to make the matrix fly from the base

Methodology Applied

Scientific EffectLaser ablation: Laser Ablation

Data Source

PatentEP3712922B1Method, device and carrier medium for preparing measurement sample for maldi mass spectrometry, measurement sample for maldi mass spectrometry, and maldi mass spectrometry method

Publication Date: 2025.09.24 RICOH CO LTD

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Figure 1B~1C
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AI summary

A method for preparing a measurement sample for MALDI mass spectrometry, the method including applying a laser beam to a base (201) containing a matrix (202) disposed on a surface of the base (201), in a manner that the laser beam is applied to a surface of the base (201) opposite to the surface on which the matrix (202) is disposed, to make the matrix (202) fly from the base (201) to be disposed at a predetermined position of an analyte (301) of MALDI mass spectrometry. A measurement sample for MALDI mass spectrometry, comprising an analyte (301) and two or more kinds of matrices (202) disposed at different predetermined positions of the analyte.