Mannanase Variants for Protease-Stable Detergent Enzymes

Resolve Bottlenecks,
Find Innovative SolutionsGenerate Solutions

Solution Overview

Problem

Existing mannanases exhibit insufficient stability under conditions relevant for detergent use, particularly in the presence of proteases, which affects their performance and usefulness in cleaning processes.

Innovation Solution

Development of mannanase variants with specific deletions and optional substitutions at defined positions, enhancing their stability and maintaining mannanase activity, including improved half-life in detergent compositions.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If existing mannanases are used in detergent compositions, then cleaning function is provided, but stability in the presence of protease is insufficient

Engineering Contradiction:

Improvestability in the presence of proteaseVSAvoidhalf life in detergent compositions

Core Design Contradiction:

ReliabilityVSDuration of action of moving object

Solution Approach 1:

The patent applies parameter changes by modifying the amino acid sequence of the mannanase enzyme through specific substitutions and deletions. The engineered variants contain substitutions at positions 485, 486, and 490-491, which alter the enzyme's structural parameters to improve its stability and resistance to protease degradation while maintaining catalytic activity.

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent applies the extraction principle by removing specific amino acid residues at positions 490-491 from the mannanase sequence. This deletion eliminates vulnerable regions that are susceptible to protease attack, thereby extending the enzyme's half-life in detergent compositions without compromising its core mannanase function.

Inventive Principle:

Principle #2Taking out (Extraction)

2Reliability

If mannanase variants with deletions and substitutions are engineered, then stability and half-life are improved, but sequence complexity increases

Engineering Contradiction:

Improvestability in detergent compositionsVSAvoidsequence complexity

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The patent systematically modifies specific parameters of the mannanase sequence at defined positions (485, 486, 490-491) to achieve improved stability. This targeted approach to parameter change allows for controlled engineering of the enzyme's properties while maintaining overall sequence simplicity and manufacturability.

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The modified mannanase variants demonstrate enhanced stability and activity in detergent environments, leading to improved cleaning performance and durability.

Implementation Method 1

endo-1,4-β-mannanases (EC 3.2.1.78), which hydrolyze the internal glycoside bonds in the mannan backbone

Methodology Applied

Scientific EffectHydrolysis: Hydrolysis

Implementation Method 2

The main enzyme type participating in the degradation of mannans are endo-1,4-β-mannanases

Methodology Applied

Scientific EffectEnzyme: Enzyme

Data Source

PatentUS20250361497A1Mannanase variants and polynucleotides encoding same

Publication Date: 2025.11.27 NOVOZYMES AS

AI summary

The present invention relates to mannanase variants with improved stability. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.