Mass Spectrometry Screening With Isotopic MRM Quantification
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Solution Overview
Problem
Direct acoustic ionization mass spectrometry is inefficient for obtaining specific, quantitative results due to the lack of separation methods, leading to detector saturation and requiring additional time-consuming preparation steps for dilution and reanalysis, which are prone to errors.
Innovation Solution
A sample processing system that combines direct sampling with a quantitative mass analyzer using label-free multiple reaction monitoring (MRM) of natural isotopic abundance, allowing for high-throughput quantitative analysis without physical dilution, by performing mass screening and subsequent quantitative analysis using product and isotopic ion transitions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If direct acoustic ionization mass spectrometry is used for high-throughput screening, then screening throughput is improved, but quantitative accuracy deteriorates due to detector saturation and lack of separation
Solution Approach 1:
The patent segments the analytical process into two distinct stages: (1) high-throughput screening using direct acoustic ionization mass spectrometry for rapid identification, and (2) targeted quantitative analysis using LC-MS/MS for precise measurement. This segmentation allows each method to operate in its optimal performance range without compromise
Solution Approach 2:
The patent introduces an intermediary decision-making step where screening results guide whether samples proceed to quantitative analysis. Samples that screen positive or require quantification are selectively transferred to the LC-MS/MS system, creating an efficient workflow that optimizes both throughput and accuracy
2Use of energy by moving object
If samples are concentrated for direct acoustic ionization mass spectrometry, then ionization efficiency is improved, but additional preparation steps are required for dilution and reanalysis
Solution Approach 1:
The patent merges the advantages of concentrated sample analysis (high ionization efficiency) with automated sample transfer capabilities. The Acoustic Droplet Ejection system automatically handles sample transfer and the workflow integrates screening and quantification steps, eliminating manual dilution and reanalysis while maintaining high ionization efficiency in the screening stage
3Measurement precision
If manual dilution and reanalysis steps are performed, then quantitative results can be obtained, but operational errors and time consumption increase
Solution Approach 1:
The patent replaces manual mechanical operations (pipetting, dilution, transfer) with automated Acoustic Droplet Ejection technology. This substitution eliminates human error in sample handling while maintaining the necessary quantitative analysis steps, thereby improving both reliability and throughput
Solution Approach 2:
The system implements self-service through automated sample transfer and workflow management. The Acoustic Droplet Ejection system automatically identifies, transfers, and prepares samples for analysis without human intervention, reducing operational errors and increasing reproducibility
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate and efficient quantification of analytes without reanalysis or dilution, reducing errors and operational time, and extending the dynamic range of the mass analyzer for analyzing samples with varying concentrations.
Implementation Method 1
ionizing the sample; monitoring, by mass spectrometry, at least one product ion transition for the at least one analyte and at least one isotopic ion transition for the at least one analyte
Implementation Method 2
acoustic radiation pressure to eject droplets from a sample plate
Data Source
AI summary
The presently claimed and described technology provides a sample processing system comprising at least one sample introduction device, wherein the at least one sample introduction device is configured to receive a sample; a mass analyzer coupled to the sample introduction device; a control system configured to at least control the at least one sample introduction device and/or the mass analyzer, wherein the mass analyzer is configured to perform a first mass analysis on the sample, wherein the first mass analysis is mass screening for an analyte of interest in the sample, and wherein if the analyte of interest is detected in the sample, the mass analyzer is configured to perform a second mass analysis, wherein the second mass analysis is a quantitative analysis, comprising: ionizing the sample; monitoring, by mass spectrometry, at least one product ion transition for the at least one analyte and at least one isotopic ion transition for the at least one analyte; determining intensity and/or abundance of the at least one product ion transition and/or the at least one isotopic ion transition; and quantifying the at least one analyte present in the sample using the intensity and/or abundance of the at least one product ion transition and/or isotopic ion transition.