Novel metalloproteases
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Solution Overview
Problem
There is a need for novel proteases suitable for specific conditions and uses in industrial applications, as existing proteases do not adequately address requirements for stability and performance in detergent compositions.
Innovation Solution
Development of novel metalloprotease enzymes derived from Bacillus species, such as Anoxybacillus rupiensis, Lysinibacillus macroides, and Brevibacillus brevis, which exhibit proteolytic activity across a range of pH and temperature conditions, making them suitable for use in detergent compositions and various cleaning applications.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If existing proteases are used in detergent compositions, then cleaning function is provided, but stability under detergent conditions is insufficient
Solution Approach 1:
The patent applies parameter changes by modifying amino acid residues at specific positions (e.g., positions 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100) in the protease sequence to enhance stability under detergent conditions while maintaining cleaning activity across different pH and temperature ranges
Solution Approach 2:
The patent creates composite enzyme formulations by combining modified protease variants with detergent components, creating a composite cleaning system that provides both stability and cleaning function. The modified proteases are designed to work synergistically with surfactants and other detergent ingredients
2Adaptability or versatility
If proteases are designed for broad pH and temperature activity, then versatility is improved, but optimization for specific conditions becomes difficult
Solution Approach 1:
The patent uses parameter changes by introducing specific amino acid substitutions at multiple positions in the protease sequence to create variants with optimized activity ranges. Each variant is tailored for specific pH and temperature conditions while maintaining overall versatility through the modular nature of the sequence modifications
Solution Approach 2:
The patent applies dynamics by creating a family of protease variants with different stability and activity profiles, allowing selection of the optimal variant for specific application conditions. The dynamic approach enables adaptation to different detergent formulations and washing conditions
3Productivity
If novel metalloproteases are developed for detergent use, then cleaning performance is improved, but development complexity increases
Solution Approach 1:
The patent systematically changes amino acid parameters at specific positions to improve cleaning performance. The structured approach to sequence modification, focusing on key regions of the protease, reduces development complexity by providing a rational design framework rather than random mutagenesis
Solution Approach 2:
The patent uses copying by creating multiple variants based on a parent sequence, allowing systematic exploration of sequence-space to improve cleaning performance. The modular variant design enables efficient screening and selection of high-performance enzymes
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The novel metalloproteases demonstrate enhanced stability and cleaning performance in detergent compositions, effectively hydrolyzing proteins in the presence of surfactants and other components, thereby improving cleaning efficacy across different conditions.
Implementation Method 1
Metalloproteases (MPs) are among the hydrolases that mediate nucleophilic attack on peptide bonds using a water molecule coordinated in the active site
Implementation Method 2
a divalent ion, such as zinc, activates the water molecule. This metal ion is held in place by amino acid ligands, usually 3 in number
Data Source
Figure 1.1~1.2
Figure 1.3~1.4
Figure 1.5A~1.5B
AI summary
Aspects of the present compositions and methods relate to novel metalloproteases polynucleotides encoding the novel metalloprotease, compositions and methods for use thereof.