Microbiological Growth Media Composition for Anaerobe and Surface Growth
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Solution Overview
Problem
Existing microbiological growth media, such as trypticase soy broth and fluid thioglycollate medium, are inadequate for supporting the growth of a wide range of microorganisms, particularly anaerobes and organisms on surfaces, limiting their effectiveness in detecting contaminants in sterile products.
Innovation Solution
A composition comprising casein digest, soybean digest, animal tissue digest, yeast extract, dextrose, phosphate buffer, hemin, and L-cystine, optionally with a gelling agent and surfactant, which supports the growth of aerobic and anaerobic bacteria, molds, and injured spores, including on membranes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If traditional growth media (TSB and FTM) are used, then general purpose growth is supported, but the ability to support anaerobic organisms and surface growth is insufficient
Solution Approach 1:
The patent uses a composite growth medium containing multiple components: casein digest, soybean digest, animal tissue digest, yeast extract, dextrose, phosphate buffer, hemin, and L-cystine. This composite formulation provides both general purpose growth support and specialized nutrients for anaerobic organisms and surface-grown microorganisms, resolving the contradiction between general adaptability and specific detection reliability.
Solution Approach 2:
The patent modifies the chemical parameters of the growth medium by incorporating specific concentrations of hemin (0.001-0.05%), L-cystine (0.01-0.5%), and phosphate buffer (pH 7.0-7.5). These parameter changes create optimal conditions for anaerobic growth and surface colonization, improving detection reliability while maintaining general versatility.
2Adaptability or versatility
If FTM is used to detect anaerobes, then anaerobic growth is possible, but the medium has limited growth promotion properties and poor surface growth support
Solution Approach 1:
The patent enhances FTM's deficiencies by adding a composite of protein digests (casein, soybean, animal tissue) and yeast extract, which provide rich growth promotion properties. The inclusion of hemin and L-cystine specifically boosts anaerobic growth capability, while the overall composite formulation enables both anaerobic growth and surface growth support simultaneously.
Solution Approach 2:
The invention creates a universal growth medium that performs multiple functions: supporting anaerobic growth, promoting aerobic growth, enabling surface growth, and providing general purpose cultivation. This multi-functionality resolves the contradiction between specialized anaerobic support and overall growth promotion efficiency.
3Productivity
If specialized media like Schaedler media are used, then specific microorganism growth is optimized, but the ability to support diverse microorganisms is limited
Solution Approach 1:
The patent creates a universal growth medium that can support diverse microorganisms including aerobes, anaerobes, yeasts, and molds. The composite formulation with multiple protein digests, yeast extract, and supplemental nutrients provides a broad spectrum of growth promotion properties, enabling the medium to function optimally for many different microorganism types simultaneously.
Solution Approach 2:
The patent adjusts the chemical parameters to create a balanced growth environment: phosphate buffer at pH 7.0-7.5 provides neutral conditions suitable for most organisms, while the specific combination of protein digests and vitamins creates optimal growth conditions for diverse microorganisms without favoring any single group.
Data Source
AI summary
The present invention features general-purpose microbiological growth media capable of supporting growth of microorganisms on membranes. The media contain casein digest, soybean digest, animal tissue digest, yeast extract, dextrose, a phosphate buffer, hemin, and L-cystine. The invention features an all-purpose microbiological growth media that can support the growth of anaerobes, molds, injured spores, and general aerobic bacteria to a greater extent than other media.