Microfluidic Platelet Detection With Integrated Centrifugal Mixing
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Solution Overview
Problem
Traditional LTA detection methods for platelet function are complex, costly, and inefficient due to the need for separate preparation of platelet poor plasma and simultaneous detection of platelet rich plasma, which cannot be performed on the same device with magnetic stirring.
Innovation Solution
A microfluidic fully-automatic platelet detection method using centrifugation and centrifugal vibration to integrate platelet rich plasma and inducing agent mixing and reaction, with optical assembly data collection, eliminating the need for additional centrifuges and magnetic stirring devices.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional LTA detection method is used with separate centrifuge and magnetic stirring device, then platelet rich plasma and platelet poor plasma detection can be performed, but device complexity increases and detection efficiency decreases
Solution Approach 1:
The patent combines the centrifugal separation function and magnetic stirring function into a single integrated device. The stirring rod is positioned within the centrifugal separation chamber, allowing both platelet poor plasma separation and platelet rich plasma stirring to occur in the same device space, eliminating the need for separate centrifuge and magnetic stirring devices.
Solution Approach 2:
The integrated device performs multiple functions: centrifugal separation to obtain platelet poor plasma, magnetic stirring of platelet rich plasma with inducer, and optical detection of aggregation. This multi-functional design reduces device complexity while maintaining detection reliability for both platelet poor plasma and platelet rich plasma.
2Reliability
If traditional LTA detection method is used with multiple separate devices, then complete detection can be performed, but detection time increases and productivity decreases
Solution Approach 1:
The integrated device enables continuous operation where platelet poor plasma is separated first, then platelet rich plasma is immediately stirred and detected without transferring samples between separate devices. The optical detection system continuously monitors the aggregation process, eliminating idle time and improving detection throughput.
Solution Approach 2:
By merging separation, mixing, and detection functions into one device, the patent eliminates the time required for sample transfer and setup between separate devices, significantly improving detection efficiency while maintaining complete detection capability.
3Reliability
If traditional LTA detection method is used with additional centrifuge equipment, then platelet poor plasma preparation is achieved, but cost increases
Solution Approach 1:
The patent integrates the centrifugal separation mechanism directly into the detection device, eliminating the need for separate centrifuge equipment. The stirring rod is positioned to allow effective separation of platelet poor plasma while maintaining structural simplicity, thereby reducing overall device cost while ensuring platelet poor plasma quality.
4Reliability
If magnetic stirring device is used separately from centrifuge, then platelet rich plasma stirring is achieved, but device complexity and cost increase
Solution Approach 1:
The patent positions the magnetic stirring rod within the centrifugal separation chamber, allowing the same device to perform both centrifugal separation of platelet poor plasma and magnetic stirring of platelet rich plasma. This integration eliminates the need for separate magnetic stirring equipment while maintaining effective mixing through the inducer.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Simplifies detection steps, reduces costs, and improves efficiency by performing both platelet rich and poor plasma detection on a single device without additional equipment, enhancing detection precision and throughput.
Implementation Method 1
centrifuging the disc to transfer the platelet rich plasma to a detection area of the disc
Implementation Method 2
centrifuging the disc to transfer the inducing agent to the detection area of the disc
Implementation Method 3
centrifugally vibrating the disc to allow the platelet rich plasma and the inducing agent to mix and react
Implementation Method 4
controlling an optical assembly to collect light transmittance data of the detection area
Implementation Method 5
centrifuging the detection area of the disc to acquire platelet poor plasma
Data Source
AI summary
The present application relates to a microfluidic fully-automatic platelet detection method and a platelet analysis and homogenization system, where the microfluidic fully-automatic platelet detection method performs a detecting and sampling process of the platelet rich plasma and a detecting and sampling process of the platelet poor plasma on the same platelet analysis and homogenization device, the entire device is small in volume, neither require arrangement of any additional centrifuge device for preparing the platelet poor plasma, nor require arrangement of any additional magnetic stirring device for stirring, the detection steps are simplified, the cost is reduced, and the detection efficiency is improved.