Microorganism Fermentation for 3-Hydroxyadipic Acid Production
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Solution Overview
Problem
Current methods for producing 3-hydroxyadipic acid using a metabolic pathway in microorganisms are inefficient, with no direct evidence of the reduction reaction from 3-oxoadipic acid to 3-hydroxyadipic acid and low productivity in reported methods, making it impractical for industrial application.
Innovation Solution
Identifying and culturing specific microorganisms such as those from the genera Escherichia, Pseudomonas, Cupriavidus, and others, which naturally produce 3-hydroxyadipic acid, using a medium with suitable carbon sources and inducers to activate the metabolic pathway for efficient production.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If chemical synthesis methods are used to produce 3-hydroxyadipic acid, then production efficiency can be improved, but process complexity and cost increase
Solution Approach 1:
The patent replaces complex chemical synthesis processes with a biological system (microorganism fermentation). The multi-step chemical synthesis involving multiple reagents, catalysts, and purification steps is substituted by a single fermentation process where microorganisms naturally produce 3-hydroxyadipic acid through metabolic pathways, significantly simplifying the overall process while maintaining high productivity
Solution Approach 2:
The patent utilizes the microorganism's endogenous metabolic capabilities to produce 3-hydroxyadipic acid. By engineering or selecting microorganisms with enhanced metabolic pathways, the system performs self-service production without requiring external chemical catalysts, complex reaction conditions, or multiple processing stages, thereby reducing process complexity while achieving efficient production
2Productivity
If existing microbial methods are used to produce 3-hydroxyadipic acid, then process simplicity is maintained, but productivity is insufficient
Solution Approach 1:
The patent employs parameter changes in the form of genetic engineering to modify microorganism characteristics. By altering genes related to metabolic pathways (such as enhancing 3-oxoadipate reductase activity or modifying substrate utilization), the microorganism's productivity for 3-hydroxyadipic acid is significantly improved while maintaining the reliability of the fermentation process
Solution Approach 2:
The patent uses microorganisms that can utilize multiple carbon sources (such as succinate, acetate, or glucose) to produce 3-hydroxyadipic acid. This multi-functionality allows the system to maintain high productivity under varying fermentation conditions while ensuring reliable and consistent production, as the microorganism can adapt to different nutrient environments
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables the effective production of 3-hydroxyadipic acid, with concentrations of at least 1.0 mg/L in the culture supernatant, confirming the microorganisms' capacity to produce the compound using their metabolic pathways.
Implementation Method 1
a method of producing 3-hydroxyadipic acid using a metabolic pathway in a microorganism
Implementation Method 2
3-oxoadipic acid (3-oxoadipate) as an intermediate in an adipic acid biosynthetic pathway is reduced by an enzymatic reaction (3-oxoadipate reductase) to allow production of 3-hydroxyadipic acid
Data Source
AI summary
A novel method of producing 3-hydroxyadipic acid using a metabolic pathway of a microorganism is disclosed. The method of producing 3-hydroxyadipic acid includes the step of culturing at least one type of microorganism having a capacity to produce 3-hydroxyadipic acid, selected from the group consisting of microorganisms belonging to the genus Escherichia, microorganisms belonging to the genus Pseudomonas, microorganisms belonging to the genus Hafnia, microorganisms belonging to the genus Corynebacterium, microorganisms belonging to the genus Bacillus, microorganisms belonging to the genus Streptomyces, microorganisms belonging to the genus Cupriavidus, microorganisms belonging to the genus Acinetobacter, microorganisms belonging to the genus Alcaligenes, microorganisms belonging to the genus Nocardioides, microorganisms belonging to the genus Brevibacterium, microorganisms belonging to the genus Delftia, microorganisms belonging to the genus Shimwellia, microorganisms belonging to the genus Aerobacter, and microorganisms belonging to the genus Rhizobium.