miRNA Plasmid Composition for CXCR2 Expression Suppression
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Over-expression or mis-expression of bioactive molecules leads to homeostatic imbalance and subsequent diseases, necessitating therapies to regulate gene expression and suppress CXCR2 molecule production.
Innovation Solution
Compositions comprising recombinant plasmids that encode miRNA sequences, administered to increase endogenous production of miRNA targeting CXCR2 mRNA, leading to its degradation or inactivation, thereby reducing CXCR2 biomolecule availability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If CXCR2 molecule production is increased to enhance immune response, then immune response capability is improved, but homeostatic balance is lost leading to disease
Solution Approach 1:
The invention changes the expression level parameter of CXCR2 by introducing miRNA sequences that specifically target and suppress CXCR2 mRNA translation, thereby reducing CXCR2 protein production to restore homeostatic balance when it is disrupted by overexpression
Solution Approach 2:
The invention uses miRNA as an intermediary molecule to mediate the suppression of CXCR2 expression. The miRNA sequences bind to complementary sequences in CXCR2 mRNA, preventing translation and thereby controlling CXCR2 protein levels without directly modifying the CXCR2 gene itself
2Quantity of substance
If miRNA production is increased to suppress target mRNA, then target biomolecule availability is reduced, but production complexity increases
Solution Approach 1:
The invention segments the therapeutic approach by separating the miRNA expression cassette from the plasmid backbone, allowing modular design and assembly. The miRNA sequence is inserted into a standardized plasmid vector containing necessary regulatory elements, simplifying the overall construction process
Solution Approach 2:
The plasmid vector is designed with universal features including origin of replication, antibiotic resistance markers, and multiple cloning sites that can accommodate different miRNA sequences. This multi-functional design allows the same plasmid backbone to be used for producing various miRNAs targeting different biomolecules
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Regulates CXCR2 expression by enhancing miRNA production, effectively addressing homeostatic imbalances and associated diseases by decreasing CXCR2 biomolecule levels.
Implementation Method 1
The sequences of miRNA may be complimentary to a sequence of target messenger RNA (mRNA) that encodes for translation of a target biomolecule and the miRNA can cause the target mRNA to be degraded or inactivated
Implementation Method 2
a backbone sequence of nucleic acids that facilitates introduction of the one or more insert sequences into one or more of a subject's cells where it is expressed and/or replicated
Data Source
AI summary
The embodiments of the present disclosure relate to one or more compositions or methods that upregulate the production of one or more sequences of micro-interfering ribonucleic acid (miRNA). The sequences of miRNA may be complimentary to a sequence of target messenger RNA (mRNA) that encodes for translation of a target biomolecule, such as CXCR2. The miRNA can cause the target mRNA to be degraded or inactivated, thereby causing a decrease in bioavailability of the target biomolecule because it is degraded or inactivated by the miRNA. Decreasing the bioavailability of the target biomolecule within a subject that is administered the one or more compositions may address the afflictions experienced by the subject due to expression of the target biomolecule.