MntH-Regulated Recombinant Microorganisms for Higher O-Phosphoserine Yield
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Solution Overview
Problem
Existing methods for producing L-cysteine and O-phosphoserine suffer from low yields, particularly in the conversion of O-phosphoserine to cysteine, necessitating excessive precursor production.
Innovation Solution
A recombinant microorganism with enhanced MntH protein activity is developed to improve O-phosphoserine production, utilizing a polynucleotide sequence with high homology to SEQ ID NO: 2, and a vector system for expression in host cells like Escherichia coli, enhancing OPS production and subsequent cysteine derivatives.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If existing non-modified strains are used for O-phosphoserine production, then the production process is simple, but the yield of O-phosphoserine and cysteine is low
Solution Approach 1:
The patent applies parameter changes by modifying the activity level of the MntH protein through genetic engineering. Specifically, the MntH gene is engineered to express a protein with enhanced manganese transport activity, which directly changes the kinetic parameter of manganese uptake. This parameter change in protein activity leads to increased intracellular manganese availability, thereby enhancing the yield of O-phosphoserine and cysteine production without requiring complete system redesign
Solution Approach 2:
The patent utilizes the universal application of the MntH protein system across different microorganism hosts. The MntH gene can be expressed in various bacterial strains (such as E. coli) to achieve enhanced manganese transport function. This multi-functional approach allows the same genetic modification strategy to be applied universally across different production strains, improving productivity while maintaining relatively simple implementation
2Productivity
If excessive amounts of O-phosphoserine precursor are produced to achieve high cysteine yield, then cysteine production increases, but the consumption of resources and production cost increase
Solution Approach 1:
The patent changes the efficiency parameter of the conversion pathway by enhancing MntH protein activity. This increases the utilization efficiency of O-phosphoserine precursor for cysteine production. By improving the manganese-dependent enzymatic steps in the pathway, less precursor is wasted, and more is efficiently converted to cysteine, thereby reducing the total quantity of precursor needed while maintaining or increasing final cysteine yield
Solution Approach 2:
The enhanced MntH activity creates a positive feedback effect where increased manganese transport improves the activity of manganese-dependent enzymes in the cysteine biosynthesis pathway. This feedback loop ensures that the metabolic flux is efficiently directed toward cysteine production, minimizing side reactions and precursor consumption, thereby optimizing resource utilization
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The recombinant microorganism achieves higher yields of O-phosphoserine and cysteine derivatives compared to non-modified strains, optimizing the production process.
Implementation Method 1
the activity of a protein having manganese importing activity is regulated
Data Source
AI summary
The present disclosure relates to a recombinant microorganism, in which the activity of a protein having manganese importing activity is regulated, and a method for producing O-phosphoserine, cysteine or cysteine derivatives using the microorganism.