mRNA Cell Conversion for Diverse Cultured Meat Tissues
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Solution Overview
Problem
Traditional meat production systems are unsustainable due to high resource consumption and greenhouse gas emissions, and cultured meat production methods lack efficiency in producing diverse cell and tissue types for heterogeneous meat products.
Innovation Solution
The use of non-human animal MYOD1 mRNA, along with other RNA molecules and colloidal lipid particles, to modulate gene expression in stem and somatic cells for differentiating or transdifferentiating them into terminally differentiated cells that produce edible meat products, including muscle, fat, and other tissues.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional livestock agriculture is used for meat production, then meat products can be produced, but resource consumption and greenhouse gas emissions are high
Solution Approach 1:
The patent replaces traditional livestock agriculture with in-vitro cell culture technology. Animal cells are cultured in bioreactors using controlled media compositions, substituting the biological processes of traditional farming with a controlled laboratory system that reduces resource consumption and eliminates greenhouse gas emissions from livestock operations
Solution Approach 2:
The patent modifies the physiological state of animal cells by controlling culture conditions, temperature, pH, and media composition to direct cell differentiation and proliferation. This allows precise control over meat product production parameters, achieving efficient meat synthesis from cellular components without the resource intensity of traditional farming
2Loss of energy
If cultured meat production uses simple cell culture methods, then resource use is reduced, but diversity of cell and tissue types is insufficient for heterogeneous meat products
Solution Approach 1:
The patent employs dynamic control of cell culture conditions to induce different differentiation pathways. By temporaly adjusting growth factors, signaling molecules, and culture media composition, the system transitions cells through multiple differentiation stages to produce diverse tissue types including muscle, fat, connective tissue, and bone within the same culture system
Solution Approach 2:
The patent uses a universal cell culture platform capable of producing multiple tissue types from the same starting cell population. The same bioreactor system and basic media formulation can generate heterogeneous meat products by modulating differentiation cues, making the culture system multi-functional for producing various meat tissue compositions
3Adaptability or versatility
If stem cell differentiation is used to produce multiple cell types, then heterogeneity of meat products is improved, but complexity of the differentiation process increases
Solution Approach 1:
The patent divides the complex differentiation process into discrete, controllable stages. Each tissue type is produced through a defined sequence of differentiation events, with specific growth factors and media conditions applied at each stage. This segmentation allows the complex process to be managed as a series of simpler, controlled transitions rather than a single complex process
Solution Approach 2:
The patent uses intermediary differentiation factors and signaling molecules to mediate the transition between cell types. These intermediaries act as controlled signals that guide stem cells through differentiation pathways, simplifying the overall process by providing discrete control points rather than requiring direct, complex transformations
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method efficiently produces a diverse range of cell types for cultured meat products, reducing resource use and emissions, and maintains cell integrity during the differentiation process.
Implementation Method 1
modulate gene expression in stem and somatic cells for differentiating or transdifferentiating them into terminally differentiated cells
Implementation Method 2
delivering one or more active agents in a colloidal lipid particle composition into said cells
Data Source
AI summary
The present invention relates to mRNA molecules and their use in differentiating cells to produce an edible meat product, as well as delivery vehicles for said mRNA molecules.