Nanomaterial-Protected dsRNA Solutions for UV-Stable Whitefly Control

The patent introduces nanomaterials (such as iron oxide nanoparticles, carbon black, or gold nanoparticles) as intermediary substances that absorb UV light and protect dsRNA from photodegradation. These nanomaterials act as mediators between the harmful UV radiation and the dsRNA, transferring the UV energy to the nanomaterials which then dissipate it as heat, thereby protecting the dsRNA structure and maintaining its RNAi activity.

The patent converts the harmful UV light into a beneficial protective mechanism by using nanomaterials that absorb UV radiation. The UV light that would normally degrade dsRNA is instead absorbed by the nanomaterials, transforming the harmful energy into heat that protects the dsRNA from photolysis, thus turning a detrimental factor into a protective one.

The patent optimizes parameters such as nanomaterial concentration (typically 0.01-10 mg/mL), dsRNA concentration, pH value (7.0-9.0), and ionic strength to achieve stable mixed solutions. By carefully controlling these parameters, the patent maintains dsRNA stability and nanomaterial dispersion while avoiding excessive complexity in the formulation process.

The patent utilizes porous nanomaterials or nanomaterials with specific surface areas that enhance dsRNA loading and protection. The porous structure provides high surface area to volume ratio, allowing efficient dsRNA attachment while maintaining solution simplicity. This approach achieves high stability without proportionally increasing formulation complexity.