Nanosecond Pulsed Electric Field Algae Aggregation

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Solution Overview

Problem

Existing methods for harvesting algae for biofuel production are inefficient due to excessive water retention, premature rupture of algae cell walls leading to lipid release, and inability to reuse algae, making them commercially unviable.

Innovation Solution

Applying nanosecond pulsed electric fields to aggregate algae without causing cell membrane disruption, allowing for efficient separation and lipid retention, eliminating the need for aggregating agents and reducing energy consumption.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If conventional harvesting methods are used to separate algae from water, then algae can be harvested, but excessive water remains in the extracted algae making lipid extraction inefficient

Engineering Contradiction:
Improvelipid extraction efficiencyVSAvoidwater content in algae
Core Design Contradiction:
ProductivityVSQuantity of substance

Solution Approach 1:

The harvesting process is segmented into distinct stages: aggregation of algae cells into flocs, separation of flocs from water, and subsequent lipid extraction. This segmentation allows each stage to be optimized independently, with aggregation achieving concentrated flocs with minimal water content, thereby improving lipid extraction efficiency

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Algae aggregation into flocs acts as an intermediary step between harvesting and lipid extraction. The floc structure serves as a water-reduced intermediate form that facilitates efficient lipid extraction while preventing cell rupture, solving the contradiction between water removal and cell integrity

Inventive Principle:
Principle #24Intermediary (Mediator)

2Productivity

If conventional harvesting methods are applied, then algae can be separated, but algae cell walls are ruptured causing premature release of lipids

Engineering Contradiction:
Improveharvesting efficiencyVSAvoidlipid loss from cell rupture
Core Design Contradiction:
ProductivityVSLoss of substance

Solution Approach 1:

The aggregation process utilizes controlled parameter changes including pH adjustment and salt addition to induce floc formation without mechanical stress that would rupture cell walls. This allows high-speed harvesting while maintaining cell integrity and preventing premature lipid release

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The aggregation step creates a protective floc structure around algae cells before the harsh conditions of lipid extraction. This pre-protection cushioning prevents cell wall rupture during subsequent processing, maintaining cell integrity throughout the workflow

Inventive Principle:
Principle #11Beforehand cushioning (Prior cushioning)

3Productivity

If conventional harvesting methods are used, then algae can be processed, but cell walls are ruptured preventing algae reuse

Engineering Contradiction:
Improveprocessing efficiencyVSAvoidalgae reusability
Core Design Contradiction:
ProductivityVSAdaptability or versatility

Solution Approach 1:

Instead of discarding algae after single-use harvesting, the gentle aggregation method preserves cell integrity allowing algae to be recovered and reused for multiple processing cycles. The floc formation and separation process maintains viable cells that can be reincubated and processed again, improving adaptability

Inventive Principle:
Principle #34Discarding and recovering

Solution Approach 2:

The aggregation-based harvesting method serves multiple functions: it enables efficient separation, preserves cell integrity for reuse, and prepares algae for various downstream applications. This multi-functionality makes the system adaptable to different biofuel production strategies and algae species

Inventive Principle:
Principle #6Universality (Multi-functionality)

4Productivity

If aggregating agents are added to facilitate algae aggregation, then separation efficiency improves, but process complexity and cost increase

Engineering Contradiction:
Improveseparation efficiencyVSAvoidprocess complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The algae system performs self-aggregation through natural physiological responses to controlled environmental changes in pH and ionic strength. This self-service aggregation eliminates the need for external aggregating agents, simplifying the process while maintaining high separation efficiency

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

Simple parameter changes in the aqueous environment (pH and salt concentration) trigger natural aggregation mechanisms in algae. This approach achieves effective aggregation without adding complex chemical agents, reducing process complexity and cost

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The method effectively aggregates algae while minimizing lipid loss, reducing the need for additional agents and energy, and enabling continuous processing for efficient biofuel production.

Implementation Method 1

The nanosecond pulsed electric field can be of an intensity and duration that the nanosecond pulsed electric field does not produce a plasma discharge or an arc discharge. The nanosecond pulsed electric fields can be applied for a duration sufficient to neutralize repulsive charges of the algae.

Methodology Applied
Scientific EffectElectrostatic repulsion neutralization: Electrostatic Induction

Implementation Method 2

while not wishing to be bound by theory and while not necessary for practicing the invention, it is believed that the nanosecond pulsed electric fields produce nanopores which allow transport of ions, such as sodium, potassium and calcium, across the algae cell membrane thereby neutralizing the repulsion between individual alga.

Methodology Applied
Scientific EffectElectroporation: Nanopore

Data Source

PatentUS8772004B2System and method for high-voltage pulse assisted aggregation of algae
Publication Date: 2014.07.08 OLD DOMINION UNIVERSITY RESEARCH FOUNDATION
  • US8772004B2 patent drawing

AI summary

A method and device for aggregating algae in an aqueous solution is disclosed. The method can include providing an algae feed comprising a liquid and algae dispersed therein. The algae feed can be aggregated by applying a nanosecond pulsed electric field to the algae feed. The nanosecond pulsed electric field can include a plurality of electric pulses having a pulse duration ranging from 1 to 1,000 nanoseconds. The method can also include separating an aggregated algae stream from the algae feed and feeding the aggregated algae stream to a lipid extraction operation.