Unicellular Algae TRIZ Cases

Supercritical carbon dioxide extracts neutral lipids from microalgal biomass in a continuous counter-current contactor.

Chemical mutagenesis modifies the cell wall of a Nannochloropsis strain to simplify extraction and reduce production costs.

Controlled electroporation and enzymatic hydrolysis improve omega-3 PUFA bioaccessibility while maintaining oxidative stability.

A dissolving solution containing nitric acid and salt enables algae to recover metals from urban mines.

Adjusting crude extract pH below 6 precipitates organic matter while retaining acid-pH-resistant phycobiliproteins in the supernatant for isolation.

A vertical tank photobioreactor system accelerates algae growth using internal LED lighting and compressed air circulation.

Reduced AcsA activity in modified microorganisms enables higher 3-hydroxypropionic acid yields by overcoming cellular toxicity during production.

Overexpressing the IDS gene in algae boosts isoprenoid yields, overcoming natural source limitations.

Bacterial conjugation delivers plasmids to diatom cells, establishing stable episomes and improving genetic manipulation efficiency.

Bacterial magnetic nanoparticles replace synthetic alternatives to separate microalgae efficiently while eliminating environmental toxicity concerns.

Shifting carbon sources from glucose to glycerol resolves the contradiction between high EPA concentration and low productivity by optimizing fatty acid ratios.

Discontinuous illumination in mixotrophic cultures reduces energy consumption while increasing polyunsaturated fatty acid production.

Applying a nanosecond pulsed electric field aggregates algae without rupturing cell walls, retaining lipids and reducing water content.

Dunaliella salina OH214 strain produces lutein pigments via photosynthesis.

Nitrogen starvation paired with intense illumination accelerates the shift from growth to lipid production, lowering chlorophyll levels for easier oil refining.

Feedback control adjusts biomass discharge to match target growth rates, resolving inefficiencies from unoptimized exhaust gas utilization.

Replacing sodium chloride with organic nutrients eliminates equipment corrosion while maintaining high DHA yields in Aurantiochytrium mangrovei cultures.

Specific apoprotein sequences retain color at pH 2.5, solving precipitation in carbonated beverages.

Flash illumination in mixotrophic Aurantiochytrium cultivation overcomes light penetration limits, boosting DHA yield beyond 30% of fatty acids.

Intermittent illumination pulses increase biomass yield and lipid content while reducing culture latency periods.

Algae biomass converts CO2 into polyacrylonitrile precursors, reducing energy consumption in carbon fiber manufacturing.

Floating substrates support microalgae biofilms to resolve the contradiction between high light exposure and collection difficulty, reducing water usage.

Dry pulverulent carbon substrate enables efficient enzymatic hydrolysis and fermentation.

Regulatory sequences and vectors overcome thick cell wall barriers to achieve consistent transgene expression in algal cells.

Chromosomal integration in recombinant algae eliminates plasmid instability and toxic by-products while reducing production costs.

Stepwise organic coagulant and inert clay addition overcomes low yield and metal contamination in microalgae harvesting.

A vector system enables stable transformation of Chlorella vulgaris via gold particle bombardment.

Suspended drilling gravel substrate enhances cyanobacteria and microalgae growth rates in culture media.

High pH flocculation concentrates algal biomass, enabling scalable extraction of lipids and sugars while resolving industrial predictability bottlenecks.

Reducing LRS2 protein expression enables green algae to withstand high-intensity light, preventing photoinhibition damage during outdoor summer cultivation.

A bioreactor system balances gas composition using pH and dissolved oxygen sensors to optimize microbial metabolism.

Diatoms buffer soil acidification and release organic matter while increasing rice yield and lodging resistance.

Viral infection replaces mechanical disruption, lowering energy consumption while maintaining high lipid yields.

Modifying nitrogen to phosphorus ratios selectively enriches polyunsaturated fatty acids without reducing total lipid yields.

Engineered ptxD enzyme resolves environmental safety concerns from antibiotic markers while maintaining high transformation efficiency.

Exogenous fungal terpene synthases produce terpenoid biocides within algal cultures to defend against rotifer predation.

Downregulating nitrate transporter genes in microalgae confers resistance to chloric acid substrate analogs.

A microorganism culture method transitions cells between phototrophic, mixotrophic, and heterotrophic conditions to optimize growth rates.

Engineered microalgal cells produce stable triglycerides using LPAAT and FATB enzymes, eliminating polyunsaturated fatty acids.

Stepwise algal cultivation achieves high lipid content through controlled nutrient ratios, reducing energy costs for biofuel production.

Culturing animal cells with unicellular algae under light produces oxygen to prevent interior necrosis in thick tissue constructs.

Floating containment devices utilize deep ocean water for algal biomass production, reducing freshwater consumption and land competition.

pH adjustment disrupts algal cell walls to expose hydrophobic lipid tails, enabling organic solvent extraction that eliminates energy-intensive centrifugation.

Navicula oiliticus microalgae produce aliphatic hydrocarbons through nutrient-restricted culture methods.

Microalgae replace petroleum binders in carbonaceous fines agglomeration, eliminating environmental pollution from persistent synthetic materials.

Specific K/N and Mg/N ratios in Schizochytrium MTCC 5890 steady-state fermentation resolve productivity-cost trade-offs.