Acid-pH-resistant Phycobiliprotein Purification via pH Adjustment
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Solution Overview
Problem
The purification of acid-pH-resistant phycobiliproteins, particularly from Galdieria sulphuraria, is complex due to the composition of the cell wall and existing methods like ammonium sulphate precipitation are costly and inefficient for industrial-scale implementation.
Innovation Solution
A process involving adjusting the pH of a crude extract to below 6 to precipitate organic matter, recovering the supernatant containing acid-pH-resistant phycobiliproteins, and isolating them, which allows for efficient separation and purification without the need for stabilizing agents.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If ammonium sulphate precipitation is used to purify phycobiliproteins, then purification can be achieved, but the process becomes costly and inefficient for industrial-scale implementation due to the need for large amounts of ammonium sulphate and subsequent reprocessing requirements
Solution Approach 1:
The invention changes the pH parameter of the crude extract to below 6, which causes organic matter to precipitate while acid-pH-resistant phycobiliproteins remain in solution. This parameter change enables selective purification without requiring large amounts of chemical precipitants like ammonium sulphate, making the process scalable for industrial production.
2Manufacturing precision
If chromatographic methods are used to achieve high purity levels, then purification quality is improved, but the implementation cost becomes prohibitively expensive
Solution Approach 1:
The invention uses pH adjustment as a simple, low-cost parameter change to achieve purification. By adjusting pH below 6, organic matter precipitates while phycobiliproteins remain soluble, enabling high purity levels to be achieved through a straightforward precipitation-filtration process rather than expensive chromatographic methods.
3Manufacturing precision
If ammonium sulphate precipitation is used, then some purification is achieved, but substantial extraction yield losses occur
Solution Approach 1:
The invention changes the pH parameter to below 6, which selectively precipitates organic matter while keeping acid-pH-resistant phycobiliproteins in solution. This selective precipitation based on pH resistance maintains high extraction yields because the target proteins are not co-precipitated with impurities, unlike ammonium sulphate methods.
4Manufacturing precision
If complex purification processes are used to remove micelles and soluble proteins, then purity is improved, but the process complexity increases
Solution Approach 1:
The invention uses a single parameter change (pH adjustment below 6) to simultaneously precipitate multiple types of impurities including micelles and soluble proteins, while leaving acid-pH-resistant phycobiliproteins in solution. This simplifies the purification process to a single precipitation-filtration step rather than multiple complex purification stages.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This process achieves high purity levels of acid-pH-resistant phycobiliproteins, specifically phycocyanins, with controlled composition, and is economically viable for industrial-scale implementation, reducing the need for costly chromatographic methods.
Implementation Method 1
adjusting the pH of the crude extract of acid-pH-resistant phycobiliproteins to a pH below 6 so as to precipitate organic matter other than acid-pH-resistant phycobiliproteins
Implementation Method 2
precipitate organic matter other than acid-pH-resistant phycobiliproteins
Implementation Method 3
recovering the supernatant comprising acid-pH-resistant phycobiliproteins
Implementation Method 4
isolating acid-pH-resistant phycobiliproteins from the supernatant
Data Source
AI summary
The present invention relates to a novel process for purifying phycobiliproteins, in particular acid-pH-resistant phycobiliproteins, the resulting phycobiliproteins, and the uses thereof.