Nerolidol Synthase Domains for Recombinant Strain Biosynthesis
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Solution Overview
Problem
Existing methods for nerolidol synthesis, such as plant extraction and chemical synthesis, face limitations including complex processes, low yield, high costs, and environmental impact due to the use of toxic reagents, necessitating a safer and more sustainable production pathway.
Innovation Solution
Development of a nerolidol synthase enzyme with domains of Pfam Nos. PF01397 and PF03936, integrated into a recombinant strain for biosynthesis, enhancing nerolidol yield through metabolic engineering and synthetic biology.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If plant extraction method is used for nerolidol synthesis, then nerolidol can be obtained from natural sources, but the process becomes complex and yield is low due to seasonal effects
Solution Approach 1:
The patent replaces the mechanical/plant-based extraction system with a biological enzymatic system. By using nerolidol synthase enzyme from microbial sources, the invention substitutes plant extraction with enzyme-catalyzed biosynthesis, eliminating seasonal dependencies and achieving consistent high-yield production regardless of plant growing conditions
Solution Approach 2:
The invention changes the fundamental production parameter from natural plant content (which varies seasonally) to controlled enzymatic reaction conditions. By optimizing enzyme concentration, substrate availability, and reaction conditions, the system achieves stable and predictable nerolidol production that is independent of seasonal variations
2Productivity
If chemical synthesis method is used for nerolidol production, then nerolidol can be produced continuously, but toxic reagents are involved which impact the environment and leave residues in products
Solution Approach 1:
The patent converts the traditional harmful chemical synthesis approach into a beneficial biosynthetic process. By using enzymatic catalysis instead of toxic chemical reagents, the invention eliminates environmental harm and product residues while maintaining continuous production capability. The enzyme system provides a green chemistry solution that transforms the harmful aspect into a safe and sustainable process
Solution Approach 2:
The invention introduces an enzymatic intermediary (nerolidol synthase) that mediates the synthesis of nerolidol without requiring toxic chemical reagents. This biological mediator enables continuous production while avoiding the harmful effects of traditional chemical catalysts, as the enzyme can be reused and does not leave toxic residues in the final product
3Reliability
If plant extraction method is used, then nerolidol is obtained from natural sources, but extraction cost is high and solvent residues remain in products
Solution Approach 1:
The patent replaces the extraction mechanism with a biosynthetic mechanism. Instead of extracting nerolidol from plant materials using solvents (which leaves residues and is costly), the invention uses nerolidol synthase enzyme to directly produce nerolidol from simpler precursors. This substitution eliminates solvent usage, reduces extraction costs, and ensures product safety by avoiding residue contamination
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The recombinant strain significantly improves nerolidol production yield, offering a safer and more efficient alternative to traditional synthesis methods.
Implementation Method 1
The invention belongs to the field of nerolidol biosynthesis, and particularly relates to nerolidol synthase and use thereof
Data Source
AI summary
Provided are a nerolidol synthetase comprising structural domains having Pfam numbers PF01397 and PF03936, and a use thereof. The amino acid sequence of the nerolidol synthase is as shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 11, or SEQ ID NO: 12, and a nucleotide sequence encoding a nucleic acid molecule is as shown in SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, or SEQ ID NO: 10. The use includes: integrating a coding gene of the nerolidol synthetase into a nucleic acid construct and introducing same into a host cell to obtain a recombinant bacterium, so that the coding gene is expressed in the recombinant bacterium. Thus, biosynthesis of nerolidol is implemented, and the yield of nerolidol is remarkably improved.