NiR Overexpression for Plant Genetic Transformation Efficiency
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Solution Overview
Problem
Current genome editing technologies face challenges in efficiently delivering gene-editing nuclease proteins or coding nucleic acids to plant cells, particularly in difficult-to-transform species like octoploid strawberry, leading to time-consuming and inefficient genetic transformation and gene editing processes.
Innovation Solution
Overexpressing Nitrite Reductase (NiR) in plant cells by introducing an expression construct containing the NiR coding nucleic acid sequence, which improves the regeneration efficiency of plant cells and enhances the transformation and gene editing efficiency by promoting nitrogen metabolism, thereby facilitating the introduction and integration of exogenous nucleic acid sequences, including gene editing systems like CRISPR.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional genetic transformation methods (agrobacterium-mediated transformation or particle bombardment) are used, then gene editing can be achieved, but the transformation efficiency is low and the process is time-consuming, especially in difficult-to-transform species like octoploid strawberry
Solution Approach 1:
The invention changes the biochemical parameters of plant cells by overexpressing NiR enzyme, which alters nitrogen metabolism and cellular physiological state. This parameter change enables cells to enter a more favorable state for regeneration and transformation, directly improving transformation efficiency and reducing the time required for successful gene editing in difficult-to-transform species
2Productivity
If genome editing is performed in difficult-to-transform plant species, then new crop varieties can be developed, but the regeneration efficiency of plant cells remains low
Solution Approach 1:
By overexpressing NiR, the invention fundamentally changes the physiological parameters of plant cells including nitrogen metabolism, cellular energy state, and regenerative capacity. These parameter changes make previously difficult-to-transform species much more amenable to genetic manipulation and regeneration, directly addressing the ease of manufacture concern
3Adaptability or versatility
If conventional breeding techniques are used, then traditional crop improvement can be achieved, but genetic diversity decreases and breeding bottlenecks occur
Solution Approach 1:
The invention replaces conventional mechanical breeding methods (crossing, selection) with precise molecular-level genome editing. This substitution allows for the creation of novel genetic variations that were previously impossible through traditional breeding, thereby maintaining and expanding genetic diversity while dramatically improving breeding efficiency and productivity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Significantly increases the regeneration efficiency of plant cells and the transformation efficiency of exogenous nucleic acid sequences, particularly in challenging species such as strawberry and wheat, leading to improved gene editing outcomes with higher yields of transformed plants.
Implementation Method 1
expression of gene NiR which promotes nitrogen metabolism in plants
Data Source
AI summary
The invention belongs to the field of genetic engineering in plants. In particular, the invention relates to a method for improving efficiency of genetic transformation and gene editing in plants. More particularly, the invention relates to a method for improving regeneration efficiency of genetic transformation and/or gene editing efficiency in plants by expression of gene NiR which promotes nitrogen metabolism in plants.