Hydrolases TRIZ Cases

An m6A-coupled effector protein expression system delivers targeted therapeutic proteins to cancer cells via specific methylation sensing.

Motif-labeled optical mapping detects off-target gene editing at 1% efficiency, avoiding costly whole genome sequencing.

Concatemer donor design segments multiple template copies via guide RNA targets to boost homology-directed repair frequency and overcome low integration rates.

Segmenting the vector removes prokaryotic sequences to eliminate immunogenicity while sustaining high expression efficiency in engineered cells.

A polymerase-free enzyme mix fragments double-stranded DNA using nickase and nuclease activity.

Multi-site genome recombination creates new genes in vivo, bypassing regulatory hurdles associated with foreign DNA insertion.

Chimeric guide RNA directs Cas9 endonuclease to cleave target DNA sequences in eukaryotic cell nuclei.

Penicillium mold cultures ripen pork without antibiotics or added salt, resolving consumer rejection of broad-spectrum treatments.

CRISPR-Cas editing of the DRO1 gene modifies root architecture, resolving limited water absorption capacity to boost crop yields.

Rational design modifies specific protein domains to increase enzymatic susceptibility, avoiding extensive screening required by random mutagenesis.

Modular CASTs enable targeted DNA integration despite limited natural diversity.

CRISPR system delivered by mitochondria-specific adeno-associated virus edits target mitochondrial DNA sequences.

A transgenic pig liver expresses human coagulation factors while suppressing porcine equivalents.

Genetic knockout of specific endogenous proteins in mammalian cell lines reduces purification complexity while maintaining high cell viability and productivity.

Human anti-TMPRSS2 antibodies bind the host protease to block viral fusion protein cleavage, bypassing drug resistance from direct viral targeting.

Enzymatic assembly of dumbbell-shaped DNA molecules yields pure double-stranded nucleic acids without solid supports or polymerase amplification.

A non-replicative DNA vector delivers genetic payloads via phage particles to modulate host microbiomes without autonomous replication.

Enzymatically modified lecithin regenerates oxidized tocopherols, overcoming thermal instability and high concentration requirements of natural antioxidants.

Reducing MHC class I and II expression while adding HLA-E, HLA-G, and PD-L1 prevents immune rejection for prolonged cell persistence.

Overexpressing nitrite reductase improves nitrogen metabolism to resolve low regeneration efficiency in difficult-to-transform plant species.

A double-gene knockout vector system targets CD163 and CD13 in porcine fibroblasts using optimized CRISPR/Cas9 components.

Direct protein introduction bypasses heat stress and time-consuming gene expression, enabling stable trait acquisition in vegetatively propagating plants.

Introducing a fluorescent marker gene alongside the target locus eliminates PCR analysis, preventing cell consumption and reducing processing time.

Duplex-specific nuclease digestion of hybridized probes releases detectable labels, enabling sensitive nucleic acid detection without amplification.

Modified nucleic acid barcodes release upon enzymatic cleavage to enable noninvasive disease monitoring.

Genetically modified cell lines reduce peptide amidation to minimize heterogeneity and immunogenicity risks while maintaining high protein yield.

Inhibiting PTPσ signaling overcomes inhibitory CSPG scars in root avulsion injuries, enabling axon growth across the lesion site.

A CRISPR-Cas system uses guide-polynucleotides to direct Cas enzymes toward specific algal DNA sequences.

Extracted Cas13d domains reduce protein size for AAV delivery while maintaining high specificity and minimizing off-target effects in human cells.

Engineered deaminases process double-stranded DNA directly, eliminating denaturation steps that complicate methylation analysis workflows.

Marker-assisted selection accelerates soybean variety XBP18007 development by tracking multiple agronomic traits simultaneously.

Prime editing guide RNA corrects FANCC mutations, reducing sensitivity to DNA-damaging agents.

Circularizing reagents select target amplicons to reduce non-target byproducts below 50% weight, resolving multiplex PCR yield trade-offs.

Recombinant UGT enzymes drive mogroside biosynthesis, bypassing low plant yields and eliminating the aftertaste from traditional extraction.

Big-IN platform enables iterative insertion of large DNA payloads into mammalian genomes using recombinase-mediated cassette exchange.

Thermosensitive enzyme-polymer conjugates switch solubility to recycle biocatalysts without solid support immobilization losses.

Deleting the catB gene via CRISPR-Cas9 creates amphenicol-sensitive Clostridium beijerinckii for industrial solvent manufacturing.

Adaptor ligation targets linear RNA free ends for magnetic bead removal, avoiding RNase-R degradation of circular transcripts.

Covalent enzyme-pore constructs eliminate amplification chemicals by detecting nucleotides via distinct ionic current blockades.

Targeting splice acceptor sites reduces non-specific cleavage risks and minimizes DNA deletions for precise exon skipping.

Mutating CD38 epitopes on non-malignant cells prevents monoclonal antibody off-target effects while maintaining protein function.

CRISPR/Cas9 mediates site-specific gene integration in immune cells, resolving random viral vector risks and preventing T cell exhaustion.

Segmented RNA-guided nuclease systems separate the enzyme from the guide RNA, reducing manufacturing complexity while maintaining high targeting specificity.

Anti-CRISPR agents inhibit CRISPR activity timing, reducing mosaicism and off-target effects by 74-79% while maintaining editing completeness.

Enzymatic demethylation and crosslinking convert coffee pulp into high-value polyphenol-functionalized pectin for food and pharmaceutical applications.

A heat-resistant mismatch endonuclease cleaves mismatched base pairs in double-stranded nucleic acids.

Region capture micro-C applies hierarchical binning to resolve fine-scale enhancer-promoter interactions while reducing sequencing costs.

Targeted amino acid modifications in lipase variants lower butyric acid release to resolve the contradiction between wash performance and residual textile odor.