NIR Fluorescent Probe for Mitochondrial Labeling
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Solution Overview
Problem
Conventional methods for labeling mitochondria using fluorescent dyes face challenges such as low transmittance due to visible light emission and nonspecific fluorescence absorption in biomolecules, making it difficult to observe fluorescence images in deep tissue effectively.
Innovation Solution
A near-infrared (NIR) fluorescent probe, represented by Chemical Formula 1, is developed to specifically label mitochondria, emitting light in the NIR range and generating red fluorescence, which is synthesized through a series of chemical reactions involving compounds like 2,4-dimethylpyrrole, 4-nitrobenzaldehyde, and pyridinecarbaldehyde, allowing for high transmittance and reduced nonspecific absorption.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional fluorescent dyes are used for labeling mitochondria, then mitochondria can be labeled, but transmittance is lowered and nonspecific fluorescence absorption occurs
Solution Approach 1:
The patent changes the emission wavelength parameter of the fluorescent probe from visible range to near-infrared (NIR) range (650-900 nm). This parameter change resolves the contradiction by enabling specific mitochondrial labeling while avoiding nonspecific fluorescence absorption, as NIR light experiences less scattering and absorption by biological tissues.
Solution Approach 2:
Instead of using conventional visible light fluorescent dyes that are readily available, the patent inverts the approach by developing probes that emit in the NIR range, where biological tissues have higher transmittance. This inversion resolves the contradiction between labeling capability and tissue penetration.
2Reliability
If visible light range fluorescent dyes are used, then mitochondria can be labeled, but it is difficult to observe fluorescence images in deep tissue
Solution Approach 1:
The patent changes the optical parameter from visible light emission to near-infrared emission (650-900 nm). This parameter change enables deep tissue imaging by exploiting the optical window in biological tissues where absorption and scattering are minimized, while maintaining specific mitochondrial labeling capability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The NIR fluorescent probe enables specific labeling of mitochondria with high transmittance, allowing for clear fluorescence imaging in deep tissue by emitting light in the NIR range and minimizing nonspecific fluorescence absorption in biomolecules.
Implementation Method 1
A near-infrared (NIR) fluorescent probe for labeling mitochondria... the fluorescent probe... may generate a red fluorescence
Data Source
AI summary
Disclosed is a fluorescent probe specifically labeling mitochondria, which can exhibit high transmittance by virtue of light emission in the NIR range and in which nonspecific fluorescence absorption in biomolecules can be avoided, making it possible to observe fluorescence images in deep tissue.