Novel Lipase for Transesterification Yield
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Solution Overview
Problem
The processing of oils and fats requires enzymatic transesterification, but existing enzymes often cause hydrolysis reactions, reducing yield and affecting the quality of oils and fats due to the production of partial glycerides and fatty acids.
Innovation Solution
A novel lipase with a high transesterification ability is identified, characterized by specific amino acid sequences, which minimizes hydrolysis reactions during transesterification, thereby improving the yield and quality of oils and fats by promoting site-non-specific transesterification.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional lipases are used for enzymatic transesterification, then the reaction can proceed, but hydrolysis side reactions occur reducing yield and quality
Solution Approach 1:
The patent applies parameter changes by modifying the amino acid sequence of the lipase enzyme to create a novel variant with enhanced transesterification activity and reduced hydrolysis activity. Specific amino acid substitutions are introduced to alter the enzyme's catalytic properties, transforming it from a conventional lipase that causes side reactions to an improved enzyme that minimizes hydrolysis while maintaining or enhancing transesterification efficiency.
2Reliability
If existing lipases are used, then enzymatic reaction occurs, but partial glycerides and fatty acids are produced adversely affecting quality
Solution Approach 1:
The patent modifies the enzyme's amino acid sequence parameters to change its catalytic selectivity. The novel lipase variant is designed to preferentially catalyze transesterification reactions over hydrolysis reactions, thereby improving the quality of oils and fats by reducing the formation of unwanted partial glycerides and fatty acids as side products.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The novel lipase effectively enhances the transesterification efficiency, reduces the production of diglycerides and fatty acids, and improves the physical properties of oils and fats, leading to higher yield and quality of triglycerides.
Implementation Method 1
a novel lipase having a transesterification ability and uses of the same
Implementation Method 2
Transesterification reactions of oils and fats are effective methods for modifying the physical properties of oils and fats
Implementation Method 3
an enzyme that has a high transesterification ability and is less likely to cause a hydrolysis reaction, which is a side reaction, during the reaction has not been put into practical use
Data Source
AI summary
To find a novel lipase suitable for processing of oils and fats and to use and apply the lipase. Provided are a novel lipase including an amino acid sequence having 90% or more identity with an amino acid sequence of SEQ ID NO: 1 or 2, and an enzyme preparation containing the same as an active ingredient. The enzyme preparation is used for modifying and improving the physical properties of oils and fats.