Hydrolases TRIZ Cases

Editing the CD123 gene in hematopoietic stem cells prevents depletion of noncancerous cells during anti-CD123 cancer therapy.

A universal lysis buffer combines chaotropic, reducing, and proteolytic agents to lyse diverse bodily samples in a single tube.

Cyclizing phytases via intein fusion resolves pelleting heat degradation while reducing gastric allergenicity through conditional stability.

Hybridized complexes form from oligonucleotide pools to assemble double-stranded DNA structures.

Direct dCas9/gRNA binding to target RNA eliminates gene isolation and amplification steps, enabling rapid pathogen identification.

Thermostable mannanase enzymes extract soluble solids from roast and ground coffee at elevated temperatures.

Measuring FGF-19, PLA2G10, and CPA2 proteins in blood samples identifies mild cognitive impairment and Alzheimer's disease markers.

Segmenting fatty acid biosynthesis via specific enzymes controls regiospecific distributions and oxidative stability in recombinant oleaginous cells.

Site-directed mutagenesis modifies lipase sequences to resist detergent interference, maintaining enzyme stability and lipid stain removal at high temperatures.

Engineered host cells express UDP-glycosyltransferase enzymes to produce mogrosides, bypassing labor-intensive extraction from natural sources.

Optimizing induction temperature and pH parameters to increase beta-lactamase production yield up to 50 grams per liter.

Membrane segmentation isolates acrylamide in a permeate stream for biocatalyst treatment, preserving retentate aroma and enabling enzyme reuse.

Conjugating a specific IgG hinge protease to an antibody extends the therapeutic half-life while preventing immune responses against the enzyme.

Engineered recombinant cells express specific thioesterase and synthetase genes to synthesize medium-chain methyl ketones.

Novel esterases use specific amino acid substitutions to enhance polyester degrading activity and thermostability.

Targeted integration links recombinant receptors to endogenous T cell stimulation loci, resolving off-target effects and antigen-independent signaling.

Peptide N-glycanase converts asparagine to aspartic acid, resolving isotopic ambiguity in glycoprotein site detection.

Disrupting SAMHD1 in iPSCs resolves viral resistance during differentiation, enabling robust functional genomics screening.

Nucleic acid-guided CRISPR nucleases complex with guide RNAs to cleave substrates with high specificity.

Rnase R degrades non-methylated RNA to identify 2'-O-methylation sites through specific hydrolysis termination patterns.

Silencing non-homologous end joining in a helper strain overcomes compartmentalization barriers to enable precise fungal genome engineering.

Directed evolution creates phosphotriesterase variants that overcome low catalytic efficiency against V-type nerve agents like VX.

Engineered amino acid sequences in this lipase minimize hydrolysis side reactions, boosting triglyceride yield and quality.

Target site blockers inhibit microRNA binding to enhance Arginase 2, resolving inflammation and tissue damage trade-offs.

Predetermined target sites enable direct exogenous DNA integration, eliminating random insertion screening and reducing pleiotropic effects.

Cysteine-modified polypeptides self-assemble into 2D crystals, reducing manufacturing complexity while enabling scalable design.

N-laurylsarcosine gradients solubilize hydrophobic insect membrane receptors, preserving biochemical activity and achieving high purity.

CRISPR/Cas9 editing targets immunoglobulin heavy chain genes to induce class switch recombination in mammalian cells.

Engineered yeast cells with reduced lanosterol synthase activity boost mogrol precursor flux, lowering extraction costs.

A solid detergent uses a modified protease to clean stains effectively while maintaining textile care at lower pH levels.

Substituting proline with leucine at position 56 of the urease accessory protein resolves production capacity limits for L-valine.

Enzymatic hydrolysis of marine algae polysaccharides yields monosugars, eliminating lignin removal costs and stabilizing raw material supply.

Engineering cell hybrids with enhanced mitochondrial phenotypes boosts protein production yields up to 15 times, resolving low culture fluid output limits.

A recombinant microorganism converts pentoses and hexoses into glycolic acid through enhanced enzymatic activities in the pentose phosphate pathway.

Targeted gene deletions in orthopoxvirus vectors reduce toxicity and immunosuppressive side effects while preserving tumor replication and killing efficacy.

A Cas12 reporter oligonucleotide with an arm-stem-loop-stem-arm structure enables collateral cleavage.

Transient co-expression of bacterial PNGase F in plants removes aberrant N-linked glycans, restoring protein functionality and uniformity.

Eliminating Alpha-actinin-3 protein expression through ACTN3 gene mutation improves feedlot efficiency and meat quality by reducing Type II fiber prevalence.

A microbial biosensor uses analyte-responsive transcription factors to modulate DNA binding affinity for sensitive detection.

Engineered multiplex CRISPR arrays combine Cas12a and Cas13 cassettes to regulate multiple target genes simultaneously.

A Corynebacterium glutamicum transformant activates the shikimate pathway and introduces phenylpyruvate decarboxylase to produce 2-phenylethanol.

A CRISPR system detects viral targets using guide RNAs and isothermal amplification at ambient temperatures.

Conjugating aromatic-cationic peptides with therapeutic molecules overcomes cell membrane permeability barriers to improve intracellular delivery.

Segmented polynucleotides hybridize into functional guides, reducing synthesis complexity and improving manufacturing quality.

Replacing chemical resolution with lipase catalysis achieves high optical purity and yield, overcoming low efficiency in traditional synthesis pathways.

Extraction-free lysis buffers and isothermal amplification resolve processing time bottlenecks while maintaining high diagnostic accuracy.

Novel esterases enhance polyethylene terephthalate degradation by improving enzyme thermostability and catalytic efficiency.

XB41B14 soybean variety uses molecular markers to introgress traits, reducing breeding time while maintaining disease resistance.

Targeting the CFTR promoter with dCas9 overcomes insufficient protein levels in cystic fibrosis.