Protease-Modified Solid Detergent for Low pH Cleaning
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Solution Overview
Problem
Solid laundry detergents with high pH values suffer from negative effects on textile care, and existing proteases used in solid laundry detergents do not provide significant advantages, especially at lower pH levels, which are desirable for better textile protection and washing performance.
Innovation Solution
A solid laundry detergent containing a protease with an amino acid sequence at least 80% identical to SEQ ID NO. 1, specifically with glutamic acid or aspartic acid at position 99, and optionally additional enzymes, formulated to operate within a pH range of 4.0 to 10 in a 1% solution at 20°C, enhancing proteolytic activity and stability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Power
If high pH values are used in solid laundry detergents, then washing performance is improved, but textile care and textile protection are negatively affected
Solution Approach 1:
The patent changes the pH parameter from conventional high values (10 or more) to lower values (4.0 to below 10, preferably 6.5 to 9.5, particularly 7.0 to 9.0). This parameter change resolves the contradiction by achieving satisfactory washing performance at lower pH levels through the use of specifically modified proteases that maintain activity in this pH range, thereby protecting textiles while still providing effective cleaning.
Solution Approach 2:
The patent applies local quality by modifying the protease enzyme at a specific location (position 99 in the amino acid sequence) with particular amino acids (E, D, N, or Q). This localized modification enables the enzyme to function effectively at lower pH values, allowing the detergent to provide strong proteolytic activity without requiring high overall pH, thus protecting textiles while maintaining washing performance.
2Object-affected harmful factors
If conventional proteases are used in solid laundry detergents with lower pH values, then textile protection is improved, but washing performance deteriorates
Solution Approach 1:
The patent changes the biochemical parameters of the protease enzyme by introducing specific amino acid substitutions at position 99. This modification alters the enzyme's pH-activity profile, enabling it to maintain high proteolytic activity at lower pH values (6.5 to 9.5), thus resolving the contradiction between textile protection and washing performance.
Solution Approach 2:
The patent creates modified versions of existing proteases by copying the basic structure and function of conventional proteases but with specific amino acid substitutions at position 99. These copied and modified enzymes retain the essential proteolytic function while gaining improved performance at lower pH levels, achieving both textile protection and effective washing.
3Power
If proteases with improved low-pH performance are used, then washing performance at lower temperatures is improved, but enzyme stability may be affected
Solution Approach 1:
The patent changes the amino acid composition parameter of the protease at position 99 to achieve optimal balance between low-temperature activity and stability. The specific amino acids (E, D, N, or Q) provide both improved proteolytic activity at lower temperatures and adequate storage stability in solid detergent formulations.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The detergent exhibits improved cleaning performance on protease-sensitive stains at lower temperatures, providing effective stain removal without the need for bleach, with enhanced proteolytic activity and storage stability.
Implementation Method 1
A protease is an enzyme that catalyzes the hydrolysis of peptide bonds and is therefore able to cleave peptides or proteins
Implementation Method 2
The protease contained in a solid laundry detergent according to the invention comprises an amino acid sequence which corresponds to that shown in SEQ ID NO: 1
Data Source
AI summary
The aim of the invention is to improve the detergent power of solid textile detergents having a pH value ranging from 4 to under 10 in a solution containing 1 wt. % in demineralized water at 20 °C. Said aim is achieved by using a protease which comprises an amino acid sequence that is at least 80% identical to the amino acid sequence set forth in SEQ ID NO. 1 and contains, at position 99 of SEQ ID NO. 1, the amino acid glutamic acid (E) or aspartic acid (D), or the amino acid asparagine (N) or glutamine (Q), or the amino acid alanine (A) or glycine (G) or serine (S).