Organic Solvent Nanofiltration for 7-Dehydrocholesterol Purification

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Solution Overview

Problem

Existing methods are ineffective in separating 7-dehydrocholesterol or 25-hydroxy-7-dehydrocholesterol and a higher molecular species formed in their synthesis, making it difficult to utilize both molecules for their intended purposes and the new compound of formula (V) for coloring applications.

Innovation Solution

A purification process using organic solvent nanofiltration (OSN) with specific membranes and conditions to separate 7-dehydrocholesterol or 25-hydroxy-7-dehydrocholesterol from the higher molecular species, allowing for their individual use in vitamin D3 synthesis and as a colorant.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Manufacturing precision

If known separation techniques are used, then the separation process is simple, but the separation efficiency between 7-dehydrocholesterol and the higher molecular species is insufficient

Engineering Contradiction:
Improveseparation efficiencyVSAvoidseparation process complexity
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The invention changes the separation parameter from conventional techniques to organic solvent nanofiltration, which operates at the molecular level. This parameter change enables effective separation of 7-dehydrocholesterol from the higher molecular species that formed during synthesis, achieving high separation efficiency without requiring complex multi-step processes

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The invention replaces conventional mechanical separation techniques with membrane-based nanofiltration. The nanofiltration membrane acts as a selective barrier that separates molecules based on size and shape at the nanometer scale, substituting complex mechanical separation operations with a more efficient membrane filtration process

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Productivity

If the mixture is not separated, then the process is simpler, but both molecules cannot be utilized for their intended purposes

Engineering Contradiction:
Improveutilization of both moleculesVSAvoidpurification process complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The invention extracts the higher molecular species from the mixture of 7-dehydrocholesterol and related compounds using nanofiltration. This extraction enables both components to be isolated and utilized for their respective purposes: 7-dehydrocholesterol for vitamin D3 synthesis and the higher molecular species as a colorant, thereby improving overall productivity

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The nanofiltration process serves multiple functions simultaneously: it separates 7-dehydrocholesterol from the higher molecular species, purifies the vitamin D3 precursor, and concentrates the colorant compound. This multi-functionality increases productivity by enabling diverse product utilization from a single synthesis mixture

Inventive Principle:
Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The process achieves high separation efficiency, enabling 7-dehydrocholesterol or 25-hydroxy-7-dehydrocholesterol to be used in vitamin D3 synthesis while the higher molecular species can be used as a colorant, with minimal contamination.

Implementation Method 1

Organic Solvent Nanofiltration (OSN) is a membrane-based separation process on a molecular level

Methodology Applied
Scientific EffectNanofiltration: Semipermeable Membrane

Implementation Method 2

subjecting the solution of step a) by organic solvent nanofiltration using a membrane; c) isolating the compound of formula (I) from the permeate obtained from step b)

Methodology Applied
Scientific EffectPermeation: Permeation

Data Source

PatentEP4139324B1Organic solvent nanofiltration of 7-dehydrocholesterol or 25-hydroxy-7-dehydrocholesterol or their oh protected forms
Publication Date: 2025.10.15 DSM IP ASSETS BV
  • EP4139324B1 patent drawingFigure 1a
  • EP4139324B1 patent drawingFigure 1b
  • EP4139324B1 patent drawing

AI summary

The present invention relates to the purification of 7-dehydrocholesterol or 25-hydroxy-7-dehydrocholesterol or their OH-protected forms, respectively. It has been found that the process using organic solvent nanofiltration allows an efficient reduction of the amount of a new higher molecular compound which is formed in a 5 new synthesis process of of 7-dehydrocholesterol or 25-hydroxy-7-dehydrocholes- terol or their OH-protected forms in small amounts. Said new higher molecular compound can be used as colorant.