Oscillospiraceae sp. LBM10036 for Caproic Acid Fermentation
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Solution Overview
Problem
Current caproic acid-producing bacteria have a narrow substrate spectrum, limiting their ability to efficiently convert naturally abundant sugars like starch, glucose, and maltose into caproic acid through one-step fermentation, and they often struggle in low pH environments common in Baijiu brewing.
Innovation Solution
The Oscillospiraceae sp. LBM10036, isolated from strong aroma-type Baijiu brewing pit mud, is capable of utilizing glucose, maltose, starch, and lactic acid as substrates to produce caproic acid efficiently, tolerating low pH environments and adapting to both new and degraded pit mud conditions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If current caproic acid-producing bacteria (e.g., C. kluyveri, Ruminococcaceae bacterium CPB6) are used, then caproic acid production is achieved, but the substrate spectrum is narrow, limiting ability to convert naturally abundant sugars like starch, glucose, and maltose into caproic acid through one-step fermentation
Solution Approach 1:
The patent applies universality by developing a caproic acid-producing bacterium capable of utilizing multiple substrates including glucose, maltose, starch, and lactic acid. The bacterium expresses multiple metabolic pathways simultaneously, allowing it to convert different carbon sources into caproic acid through one-step fermentation, thereby achieving both broad substrate spectrum and high productivity.
2Reliability
If caproate-producing bacteria are used in low pH environments (common in Baijiu brewing), then caproic acid production continues, but bacterial growth and metabolism are inhibited by acidic conditions
Solution Approach 1:
The patent applies parameter changes by optimizing the bacterium's physiological parameters to tolerate low pH conditions. The bacterium has been selected or engineered to maintain optimal growth and metabolic activity at acidic pH levels typical of Baijiu brewing environments, allowing stable caproic acid production without significant inhibition of growth or metabolism.
3Productivity
If existing caproic acid-producing bacteria are used, then caproic acid is synthesized, but chemical pH adjustments are required to maintain optimal fermentation conditions, increasing process complexity and cost
Solution Approach 1:
The patent applies self-service by utilizing the bacterium's inherent ability to tolerate and thrive in low pH environments without requiring external pH control systems. The bacterium naturally adapts to acidic conditions, eliminating the need for chemical pH adjustments and simplifying the fermentation process while maintaining high caproic acid synthesis rates.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Oscillospiraceae sp. LBM10036 effectively synthesizes caproic acid across various substrates, enhances pit mud quality, and improves Baijiu production by growing vigorously in low pH conditions, reducing the need for chemical pH adjustments and simplifying fermentation processes.
Implementation Method 1
microbial fermentation is a promising anaerobic microbial fermentation technology. Caproic acid can be obtained by chemical synthesis or microbial fermentation. Due to the fact that microbial fermentation can use urban waste or industrial by-products as raw materials for the synthesis of caproic acid
Data Source
AI summary
The disclosure discloses Oscillospiraceae sp. LBM10036 capable of utilizing multiple substrates and application thereof, and belongs to the fields of microbial technology and Baijiu brewing. The microorganism Oscillospiraceae sp. LBM10036 of the disclosure was preserved in the Guangdong Microbial Culture Collection Center on Sep. 21, 2020, with the preservation number GDMCC No. 61202. The Oscillospiraceae sp. LBM10036 of the disclosure has the capability to utilize diverse substrates and high product specificity, adapts to low pH, and can efficiently synthesize caproic acid under a pH of 5.0-5.5. The strain used in the disclosure can be used for strengthening microorganisms in pit mud, and improving the quality of degraded pit mud, to improve the quality of Strong aroma-type Baijiu, and also can be used for producing caproic acid from a variety of sugars and lactic acid by microbial fermentation.