3D Particle Measurement Sensitivity Correction by Light Spot Position
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Solution Overview
Problem
Existing particle measurement technologies face challenges in maintaining consistent detection sensitivity due to individual apparatus characteristics, such as variations in emission angle, coherence length, and objective lens mounting, leading to fluctuations in detection signals when measuring microparticles undergoing Brownian motion in a solvent.
Innovation Solution
A particle measurement apparatus that corrects detection sensitivity fluctuations by using a correction function based on the irradiation position of the light spot in the X, Y, and Z directions, employing polynomial and Gaussian distribution models to account for individual apparatus characteristics.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If three-dimensional scanning of light spot is performed to measure particles, then measurement capability is improved, but detection sensitivity fluctuations occur due to individual apparatus characteristics
Solution Approach 1:
The invention introduces a detection sensitivity correction function that adjusts measurement parameters based on apparatus characteristics. By varying correction parameters (a, b, c, d) in the correction function f(x, y, z) = a·x² + b·y² + c·z² + d, the system compensates for fluctuations in detection sensitivity caused by individual apparatus characteristics such as emission angle and coherence length variations.
Solution Approach 2:
The invention implements a feedback mechanism where measurement results are used to determine and update correction parameters. The apparatus determines apparatus characteristics through measurement of standard particles, calculates correction parameters based on these characteristics, and applies them to correct detection signals. This closed-loop feedback ensures consistent measurement accuracy across different apparatuses.
2Measurement precision
If light spot scanning is performed to obtain three-dimensional image data, then particle size measurement is enabled, but fluctuations in light spot energy and interference intensity occur
Solution Approach 1:
The invention modifies the detection signal parameters by applying position-dependent correction factors. The correction function f(x, y, z) adjusts the detection signal magnitude and phase based on the light spot position, compensating for variations in light spot energy and interference intensity that occur during three-dimensional scanning.
Solution Approach 2:
The invention performs preliminary calibration by measuring standard particles with known characteristics before actual measurements. This preliminary action determines apparatus-specific characteristics and correction parameters in advance, allowing the system to pre-compensate for light spot energy fluctuations during subsequent particle measurements.
3Productivity
If individual apparatus characteristics are not corrected, then measurement speed is maintained, but measurement consistency across multiple apparatuses deteriorates
Solution Approach 1:
The invention enables each apparatus to automatically determine its own characteristics and apply self-correction. Each apparatus measures standard particles to determine its unique apparatus characteristics, calculates appropriate correction parameters, and applies them to its measurements. This self-service approach maintains measurement speed while ensuring consistency across multiple apparatuses without requiring external intervention.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The apparatus effectively reduces detection sensitivity fluctuations, ensuring precise measurement of particle size and concentration by minimizing variations across different apparatuses, thereby improving measurement accuracy and consistency.
Implementation Method 1
irradiating the particles with light, and corrects the size of the particles using a correction function
Implementation Method 2
Protein aggregates are suspended in a solvent and their position changes over time due to Brownian motion
Implementation Method 3
measuring an object to be tested whose size is approximately three times or less the size of the light spot, the optical measurement method including: a signal acquisition step of detecting reflected light reflected from the object to be tested
Implementation Method 4
detecting reflected light reflected from the object to be tested by irradiating the object to be tested with the light
Implementation Method 5
receiving the interference between signal light and interference light with four detectors with different phase conditions
Implementation Method 6
the optical measurement method including: a signal acquisition step of detecting reflected light reflected from the object to be tested
Data Source
AI summary
A particle measurement apparatus of the invention measures the size of particles in a liquid sample by irradiating the particles with light, and corrects the size of the particles using a correction function that uses as an input value the irradiation position of the light spot in at least one of the X, Y and Z directions.